803480
PDPH (3-(2-pyridyldithio)propionyl hydrazide)
別名:
3-(2-Pyridyldithio)propionate hydrazide, 3-[(2-Pyridyl)dithio]propionohydrazide
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
50 mg | カートの在庫状況を確認する | ￥40,000 |
この商品について
実験式（ヒル表記法）:
C8N3S2OH11
CAS番号:
分子量:
229.32
NACRES:
NA.22
PubChem Substance ID:
UNSPSC Code:
12352125
MDL number:
Assay:
≥95%
Form:
powder
assay
≥95%
Quality Segment
form
powder
mol wt
229.32
reaction suitability
reagent type: cross-linking reagent
storage condition
desiccated
solubility
DMSO or DMF: soluble
functional group
hydrazide
shipped in
ambient
storage temp.
2-8°C
SMILES string
NNC(CCSSC1=NC=CC=C1)=O
InChI
1S/C8H11N3OS2/c9-11-7(12)4-6-13-14-8-3-1-2-5-10-8/h1-3,5H,4,6,9H2,(H,11,12)
InChI key
NITXODYAMWZEJY-UHFFFAOYSA-N
General description
The PDPH is a heterobifunctional crosslinker containing sulfhydryl-reactive pyridyldithiol and carbonyl-reactive hydrazide moieties. Pyridyldithiols react with free sulfhydryls (-SH) to form disulfide bonds. Hydrazide groups react with carbonyls (aldehydes and ketones) to form stable hydrazone bonds. Aldehyde groups can be created by periodate-oxidation of sialic acid and other sugar components of glycoprotein polysaccharides. Thus, PDPH is useful for conjugating glycoproteins and sulfhydryl-containing peptides or proteins. Likewise, PDPH is useful as a sulfhydryl-addition reagent for glycoproteins and other carbohydrates; after reaction of the hydrazide to an oxidized carbohydrate, the pyridyldithiol group can be cleaved by a reducing agent to expose a sulfhydryl group. Yet another application for PDPH is to react the primary amine of the hydrazide moiety to a carboxyl group using the crosslinker EDC.
Features and Benefits
- Reactive groups: pyridyldisulfide and hydrazide
- Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
- Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
- Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
Disclaimer
This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.
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当該品目 | |||
|---|---|---|---|
| assay ≥95% | assay ≥95% | assay ≥90% | assay ≥90% |
| reaction suitability reagent type: cross-linking reagent | reaction suitability reagent type: cross-linking reagent | reaction suitability reagent type: cross-linking reagent | reaction suitability reagent type: cross-linking reagent |
| solubility DMSO or DMF: soluble | solubility acetone: 50 mg/mL, DMF: soluble | solubility soluble (>95 mg/ml in water) | solubility DMSO or DMF: soluble |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| form powder | form powder | form powder | form powder |
![Sulfo-LC-SPDP (sulfosuccinimidyl 6-[3′-(2-pyridyldithio)propionamido]hexanoate)](/deepweb/assets/sigmaaldrich/product/structures/266/633/e2a263be-4bd3-4fcf-89c4-75b5e2bd829c/640/e2a263be-4bd3-4fcf-89c4-75b5e2bd829c.png)
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable