Elution with less than 200 µL is possible to obtain more concentrated DNA. However, using a smaller volume may reduce the total DNA yield because the membrane might not be fully submerged, leading to less efficient elution.
G1N70
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 70 purifications
Synonym(s):
Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship TODAYfromMILWAUKEE | $272.00 |
About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
$272.00
Available to ship TODAYDetails
usage
sufficient for 70 purifications
Quality Segment
technique(s)
DNA purification: suitable
input
mammalian cells
mammalian tissue
test parameters
sample volume: 25 mg tissue, sample volume: 2x10^6 cells
storage temp.
15-25°C
General description
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
Application
The purified genomic DNA is ready for immediate use in downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Biochem/physiol Actions
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description sufficient for 70 purifications | description sufficient for 350 purifications | description sufficient for 10 purifications | description sufficient for 10 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - | technique(s) - |
| usage sufficient for 70 purifications | usage sufficient for 350 purifications | usage sufficient for 10 purifications | usage sufficient for 10 purifications |
| test parameters sample volume: 2x10^6 cells, sample volume: 25 mg tissue | test parameters sample volume: 25 mg tissue, sample volume: 2x10^6 cells | test parameters - | test parameters - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| input mammalian cells | input mammalian cells | input - | input - |
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Signal Word
Danger
Target Organs
Respiratory system
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
WGK
WGK 3
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Storage Class
10 - Combustible liquids
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Can I elute with less than one 200ul for more concentrated DNA?
1 answer-
Helpful?
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Will the kit also isolate mitochondrial DNA?
1 answer-
R&D confirms that you will obtain mtDNA in addition to genomic DNA when following a standard protocol.
Helpful?
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