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L5283

Leukemia Inhibitory Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonym(s):

Human LIF, Human Leukemia Inhibitory Factor, hLIF, LIF, rLIF

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Size/SKUAvailabilityPrice
10 μg

Available to ship TODAYfromMILWAUKEE

$452.00
5 x 10 μg
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$2,100.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00163950
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
buffered aqueous solution (pH 7.4)

$452.00

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Product Name

Leukemia Inhibitory Factor human, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

recombinant

expressed in E. coli

Quality Segment

200

assay

≥95% (SDS-PAGE)

form

buffered aqueous solution (pH 7.4)

potency

0.1-1.5 ng/mL ED50/EC50

quality

endotoxin tested

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline containing 0.02% TWEEN® 20.

Analysis Note

The proliferative activity of human LIF is measured in culture using the human leukemic cell line, TF-1.

Legal Information

TWEEN is a registered trademark of Croda International PLC

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description

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

description

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

description

10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

description

5 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

form

buffered aqueous solution (pH 7.4)

form

buffered aqueous solution

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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