The particular strain of E. coli is not disclosed for this item.
MBD0017
Inactivated Escherichia coli
Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Available to ship TODAYfromMILWAUKEE | $334.00 |
About This Item
NACRES:
NA.51
UNSPSC Code:
41105500
Quality Segment
form
liquid
concentration
>10^8 bacteria/ml
technique(s)
DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable
suitability
suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions
shipped in
dry ice
storage temp.
−20°C
General description
Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Escherichia coli standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E.coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.
Read here how to use our standards to ensure data integrity for your microbiome research.
Read here how to use our standards to ensure data integrity for your microbiome research.
Application
Inactivated bacteria are provided at >10^8 bacteria/mL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS
Suitable for Quantitative standard for PCR, Sequencing and NGS
Features and Benefits
- Individual microbial standard for microbiomics and meta-genomics workflow
- Suitable standard for PCR, sequencing and NGS
- Improve Bioinformatics analyses
- Increases reproducibility
- Compare results lab to lab
Physical form
Liquid -The inactivated bacteria is provided at >10^8 bacteria/mL in TE buffer pH 8.0
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This Item | |||
|---|---|---|---|
| description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/vial | description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| form liquid | form liquid | form powder | form liquid |
| suitability suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions | suitability - | suitability - | suitability - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) PCR: suitable, DNA extraction: suitable, DNA sequencing: suitable | technique(s) DNA extraction: suitable, PCR: suitable, DNA sequencing: suitable | technique(s) DNA sequencing: suitable, PCR: suitable, DNA extraction: suitable | technique(s) DNA sequencing: suitable, PCR: suitable, DNA extraction: suitable |
| concentration >10^8 bacteria/ml | concentration >10^8 bacteria/ml | concentration >10^8 bacteria/vial | concentration >10^8 bacteria/ml |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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What particular strain of E. coli is supplied with this item?
1 answer-
Helpful?
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Can you provide details on how the bacteria were inactivated? Were they chemically denatured or heat inactivated?
1 answer-
The inactivation method is proprietary, and sterility testing is conducted to ensure that complete inactivation has been achieved.
Helpful?
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What is the concentration of the bacteria in the suspension?
1 answer-
The number of inactivated bacteria per vial is estimated by the CFU count of the untreated control and not by direct count of the final product. The > 10x8 number indicated is an estimation of the number of inactivated bacterial cells per vial.
Helpful?
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