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MBD0018

Microbial DNA standard from Bacillus subtilis

Suitable for PCR, Sequencing and NGS, 10 ng/μL

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Size/SKUAvailabilityPrice
0.3 μg

Estimated to ship onNovember 02, 2026fromMILWAUKEE

$294.00

About This Item

NACRES:
NA.24
UNSPSC Code:
41105500

$294.00

Estimated to ship onNovember 02, 2026Details

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Quality Segment

200

form

liquid

concentration

10 ng/μL

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

shipped in

ambient

storage temp.

−20°C

General description

Microbial DNA standard from Bacillus subtilis (B. subtilis) is a genomic DNA microbial standard that can serve as a standard for benchmarking the performance, along the workflow of microbiomics or meta-genomics analyses. It can also be used as a tool to increase reproducibility and allow comparison of results obtained by different labs. Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Bacillus subtilis is a gram positive, aerobic bacterium, rod-shaped, endospore-forming, catalase-positive. It is traditionally considered as a soil microorganism. Bacillus subtilis is genetically well characterized and serves as a model organism for important gram-positive pathogens such as Bacillus anthracis, Staphylococcus aureus etc. B. subtilis possess an extreme adaptation ability to various environmental stress conditions by modifying its motility, uptake of exogenous DNA, biofilm formation and sporulation. Recent studies have identified and characterized B. subtilis isolates from human gastrointestinal track suggesting B. subtilis as a gut commensal. Moreover, B. subtilis presence in the gut was found to correlate with probiotic effects such as anti-diarrhea effect, anti-microbial effect, immune-stimulatory effect, competition with pathogens, normal flora growth stimulation and prevention of intestinal inflammation. B. subtilis beneficial effect on gut inflammation may also suggest it as potential probiotic treatment for inflammatory bowel diseases (IBD), including Crohn′s and ulcerative colitis. In addition, it was demonstrated that a peptide produced by B. subtilis possess antiviral effect against influenza virus.




Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Other Notes

It is recommended to avoid freeze thaw cycles of this product.

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description

Suitable for PCR, Sequencing and NGS, 10 ng/μL

description

Suitable for PCR, sequencing and NGS, 10 ng/μL

description

Suitable for PCR, sequencing and NGS, 10 ng/μL

description

Suitable for PCR, sequencing and NGS, 10 ng/μL

technique(s)

DNA extraction: suitable, PCR: suitable, DNA sequencing: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

concentration

10 ng/μL

concentration

10 ng/μL

concentration

10 ng/μL

concentration

10 ng/μL

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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