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Nerve Growth Factor-7S from murine submaxillary gland

Name: Nerve Growth Factor-7S from murine submaxillary gland
Brand: SIGMA
Price: 343 USD
Availability: InStock

lyophilized powder, suitable for cell culture

Synonym(s):

NGF-7S

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Size/SKU	Availability	Price
0.1 mg	Check Cart for Availability	$343.00

About This Item

CAS Number:

93928-24-6

UNSPSC Code:

12352202

NACRES:

NA.77

EC Number:

210-123-3

MDL number:

MFCD00147703

Biological source:

mouse

Form:

lyophilized powder

Mol wt:

protein 130 kDa

Impurities:

≤10

$343.00

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Properties

Product Name

Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Segment

200

form

lyophilized powder

potency

2-250 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

protein 130 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

≤10

color

white

UniProt accession no.

P01139

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

Description

General description

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α₂β₂γ₂ complex, where β₂-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.

Application

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-

PC12
Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

Biochem/physiol Actions

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.

Physical form

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

Preparation Note

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

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This Item	N5415	E1257	E5160
Sigma-Aldrich N0513 Nerve Growth Factor-7S from murine submaxillary gland	Sigma-Aldrich N5415 Nerve Growth Factor-β human	Sigma-Aldrich E1257 Epidermal Growth Factor from murine submaxillary gland	Sigma-Aldrich E5160 Epidermal Growth Factor from mouse
description NGF-7S, lyophilized powder, suitable for cell culture	description recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture	description lyophilized powder, BioReagent, suitable for cell culture	description EGF
biological source mouse	biological source human	biological source mouse submaxillary glands	biological source mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
mol wt protein 130 kDa	mol wt protein calculated mol wt 13.5 kDa (Monomer)	mol wt -	mol wt ~6 kDa

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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