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Anti-β-tubulin antibody, Mouse monoclonal

Name: Anti-β-tubulin antibody, Mouse monoclonal
Brand: SIGMA
Price: 525 USD
Availability: InStock

clone 2-28-33, purified from hybridoma cell culture

Synonym(s):

Anti-TUBB1

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$525.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

2-28-33, monoclonal

Application:

IF, IHC

Citations:

$525.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2-28-33, monoclonal

form

buffered aqueous solution

mol wt

~50 kDa

species reactivity

dog, chicken, rat, bovine, monkey, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract, immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells, immunohistochemistry: suitable

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068)
mouse ... Tubb3(22152)

Description

General description

Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.

Immunogen

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-β-tubulin has been used:

in immunoblotting
in immunofluorescence
in immunohistochemistry

Biochem/physiol Actions

Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	T5293	T8328	SAB4200732
Sigma-Aldrich SAB4200715 Anti-β-tubulin antibody, Mouse monoclonal	Sigma-Aldrich T5293 Monoclonal Anti-β-Tubulin antibody produced in mouse	Sigma-Aldrich T8328 Anti-β-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich SAB4200732 Anti-β-Tubulin I antibody, Mouse monoclonal
description clone 2-28-33, purified from hybridoma cell culture	description clone 2-28-33, ascites fluid	description ~2.0 mg/mL, clone AA2, purified from hybridoma cell culture	description clone SAP.4G5, purified from hybridoma cell culture
clone 2-28-33, monoclonal	clone 2-28-33, monoclonal	clone AA2, monoclonal	clone SAP.4G5, monoclonal
species reactivity dog, chicken, rat, bovine, monkey, human, mouse	species reactivity Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human	species reactivity human, mouse, rat, bovine	species reactivity bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey
Gene Information human ... TUBB(203068) mouse ... Tubb3(22152)	Gene Information human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) rat ... Tubb2(29212), Tubb2c(296554)	Gene Information human ... TUBB(203068)	Gene Information human ... TUBB(203068)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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