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SAB4200738

Anti-FITC antibody, Mouse monoclonal

clone FL-D6, purified from hybridoma cell culture

Synonym(s):

Anti-Fluorescein isothiocyanate

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100 μL
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$552.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
FL-D6, monoclonal
Technique(s):
immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC
Application:
ELISA (i), IHC
Citations:
2

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biological source

mouse

Quality Segment

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

FL-D6, monoclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).

Immunogen

FITC-BSA conjugate

Application

Anti-FITC antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry

Biochem/physiol Actions

FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-FITC antibody, Mouse monoclonal clone FL-D6, purified from hybridoma cell culture

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Anti-FITC antibody, Mouse monoclonal

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F5897

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

Anti-Human IgG (γ-chain specific)−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

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Anti-Human IgG (γ-chain specific)−FITC antibody produced in goat

IgG−FITC from human serum ~20 mg/mL, buffered aqueous solution

Sigma-Aldrich

F9636

IgG−FITC from human serum

description

clone FL-D6, purified from hybridoma cell culture

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

~20 mg/mL, buffered aqueous solution

biological source

mouse

biological source

goat

biological source

goat

biological source

-

clone

FL-D6, monoclonal

clone

polyclonal

clone

polyclonal

clone

-

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

-

technique(s)

indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC, immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable

technique(s)

indirect immunofluorescence: 1:64, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

direct immunofluorescence: 1:32

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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