SHC004V
MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles
shRNA sequence targeting tGFP
Synonym(s):
MISSION®, MISSION® Control Transduction Particles
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| Size/SKU | Availability | Price |
|---|---|---|
1 pkg | Check Cart for Availability | $452.00 |
About This Item
Quality Segment
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
General description
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description shRNA sequence targeting tGFP | description shRNA sequence targeting tGFP | description High Titer, shRNA sequence targeting tGFP | description Green fluorescent protein marker to monitor transduction efficiency |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) - | technique(s) capture ELISA: 109 TU/mL using p24 | technique(s) capture ELISA: 106 TU/mL using p24 |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x109 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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