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SHC016H

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer

Targets no known genes from any species

Synonym(s):

MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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1 ea
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$3,130.00

About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x109 VP/ml (via p24 assay)

$3,130.00


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Quality Segment

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

shipped in

dry ice

storage temp.

−70°C

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
High titer virus is useful when performing in vivo research.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
SHC016VSHC016SHC216V
description

Targets no known genes from any species

description

Targets no known genes from any species

description

Targets no known genes from any species

description

Targets no known genes from any species

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

≥1x106 VP/ml (via p24 assay)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100


Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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