The 'type' is a historical designation used to differentiate the various Urease products and generally indicates a specific method of isolation and/or purification. The Type IX undergoes dialysis against deionized water prior to lyophilization. The process is EDTA-free, but may leave traces of potassium phosphate buffer salts. This item has an activity range of 50000 - 100000 units per gram solid. The mass of the 20KU option may vary from 200 mg – 400 mg per vial. The lot specific activity is published in the Certificate of Analysis.
U4002
Urease from Canavalia ensiformis (Jack bean)
Type IX, powder, 50,000-100,000 units/g solid
Synonyme(s) :
Jack bean urease, Urea amidohydrolase
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| À vous/Référence | Disponibilité | Prix |
|---|---|---|
20000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 131,00 $ |
100000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 415,00 $ |
500000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 1 230,00 $ |
1000000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 2 340,00 $ |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
50,000-100,000 units/g solid
131,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aidertype
Type IX
Quality Segment
form
powder
specific activity
50,000-100,000 units/g solid
mol wt
~544620 Da
storage temp.
2-8°C
General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.
Application
Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.[1]
Biochem/physiol Actions
In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline. [2]
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.
Other Notes
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
1 of 1
Cet article | |||
|---|---|---|---|
| description Type IX, powder, 50,000-100,000 units/g solid | description Type III, powder, 15,000-50,000 units/g solid | description Type C-3, powder, ≥600,000 units/g solid | description Type III, glycerol solution, 500-800 units/mL |
| specific activity 50,000-100,000 units/g solid | specific activity 15,000-50,000 units/g solid | specific activity ≥600,000 units/g solid | specific activity - |
| form powder | form powder | form powder | form glycerol solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt ~544620 Da | mol wt ~544620 Da | mol wt ~544620 Da | mol wt ~544620 |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| type Type IX | type Type III | type Type C-3 | type Type III |
Still not finding the right product?
Explore all of our products under Urease from Canavalia ensiformis (Jack bean)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
-
Hi there I was wondering what Type IX means and how many grams are there in the 20 kU one?
1 answer-
Helpful?
-
-
Good morning, I would like to know what the difference is between the different types of urease, since some are type III, type IX, type C-III.
1 answer-
The type designations for urease products are not indications of particular isoforms. They are internal codes that refer to the chronological order of the products' market entry and manufacturing processes. The manufacturing processes are proprietary. However, the major differences between the urease products are their specifications for specific activity. Please access the 'Specification Sheet' and 'Sample Certificate of Analysis' for each individual product via its corresponding website.
Helpful?
-
-
Hi there I was wondering what Type IX means and how many grams are there in the 20 kU one?
1 answer-
The 'type' is a historical designation used to differentiate various Urease products. Each lot has a specific activity of 50,000 - 100,000 units per gram solid. Therefore, the mass of the 20 kU option can vary between 0.2 g and 0.4 g. The lot-specific certificate of analysis will provide the specific activity.
Helpful?
-