02-101
Endothelial Cell Growth Supplement, 50mg
The Endothelial Cell Growth Supplement is designed for use as a cell culture media supplement. This cell growth supplement is available in a 50 mg format & has been optimized & validated for cell culture.
Synonym(s):
Primary Cell Culture Supplement
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.77
eCl@ss:
32160801
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form
liquid
manufacturer/tradename
Millipore, Upstate®
technique(s)
cell culture | mammalian: suitable
input
sample type neural stem cell(s)
sample type epithelial cells
shipped in
dry ice
Quality Level
storage temp.
-10 to -25°C
1 of 4
This Item | |||
|---|---|---|---|
| form liquid | form liquid | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in wet ice |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level - |
| manufacturer/tradename Millipore | manufacturer/tradename Cell Applications, Inc | manufacturer/tradename - | manufacturer/tradename - |
| input sample type neural stem cell(s) | input - | input - | input - |
General description
The Endothelial Cell Growth Supplement (ECGS) is designed for cell culture media supplement. This cell growth supplement ECGS is a mitogenic and is applicable for culturing mammalian, human endothelial cells, avian, smooth muscle cells, melanocytes, keratinocytes, and hybridomas either under reduced or serum-free conditions. It acts as a substitute for feeder layers in fastidious cell cultures.
Application
Endothelial Cell Growth Supplement, 50mg has been used to culture human umbilical vein endothelial cells (HUVECs).[1]
Physical form
lyophilized from sterile water containing trace amounts of NaCl and streptomycin sulfate
Preparation Note
Lyophilized: 2 years at 4°C; rehydrated: 3 months at -20°C.
Analysis Note
routinely evaluated in a 5 day growth assay of fetal bovine heart endothelial cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk
WGK 2
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A T Kovala et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(15), 2486-2494 (2000-12-02)
The definition of signaling pathways in endothelial cells has been hampered by the difficulty of transiently transfecting these cells with high efficiency. This investigation was undertaken to develop an efficient technique for the transfection of endothelial cells for functional analyses.
N A Lanson et al.
Nucleic acids research, 28(15), 2882-2892 (2000-07-25)
To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and
S Strömblad et al.
The Journal of clinical investigation, 98(2), 426-433 (1996-07-15)
Induction of p53 activity in cells undergoing DNA synthesis represents a molecular conflict that can lead to apoptosis. During angiogenesis, proliferative endothelial cells become apoptotic in response to antagonists of integrin alphavbeta3 and this leads to the regression of angiogenic
H Hoshi et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 22(1), 51-56 (1986-01-01)
Endothelial cells from autopsy and biopsy specimens from a variety of adult human vascular tissue were harvested by collagenase treatment and gentle swabbing of the lumenal surface. Nutrient medium MCDB 107 containing a partially purified brain-derived growth factor (5 micrograms/ml)
U M Vischer et al.
Blood, 91(1), 118-127 (1998-02-07)
von Willebrand factor (vWF) is stored and released from endothelial secretory granules called Weibel-Palade (WP) bodies. Acute release can be induced by thrombin, histamine, and other mediators of thrombosis or inflammation. Their effect is thought to be mediated by an
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