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07-2189

Anti-CAPZIP Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

RCSD domain containing 1, capZ-interacting protein, Protein kinase substrate CapZIP, protein kinase substrate MK2S4, RCSD domain-containing protein 1

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Pack SizeSKUAvailabilityPrice
100 μL
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PLN 1,640.00

About This Item

eCl@ss:
32160702
UNSPSC Code:
12352203
NACRES:
NA.84
Clone:
polyclonal
Species reactivity:
human
Application:
IP, WB
Citations:
-

PLN 1,640.00

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biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_443094

UniProt accession no.

Q6JBY9

shipped in

wet ice

Gene Information

human ... RCSD1(92241)

General description

CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.
~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa

Immunogen

GST-tagged recombinant protein corresponding to human CAPZIP.

Application

Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.
Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate
Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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AB6016SAB1403633ABC346
Anti-CAPZIP Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

07-2189

Anti-CAPZIP Antibody

Anti-F-actin-capping protein subunit alpha-1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

AB6016

Anti-F-actin-capping protein subunit alpha-1 Antibody

Monoclonal Anti-CAPZB, (C-terminal) antibody produced in mouse clone 1H1, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1403633

Monoclonal Anti-CAPZB, (C-terminal) antibody produced in mouse

Anti-FAF1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABC346

Anti-FAF1 Antibody

clone

polyclonal

clone

polyclonal

clone

1H1, monoclonal

clone

polyclonal

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

pig, mouse, human, rat

species reactivity

human

species reactivity

rat, human

UniProt accession no.

Q6JBY9

UniProt accession no.

P52907

UniProt accession no.

P47756

UniProt accession no.

Q9UNN5

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
07-218904053252629457

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