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16-201

Protein G Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

Synonym(s):

ChIP agarose beads, ChIP agaraose G beads

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Size/SKUAvailabilityPrice
2.5 mL
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$463.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid

$463.00

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Quality Segment

100

form

liquid

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

General description

Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Packaging

2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.

Physical form

2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.

Preparation Note

1 year at 4°C from date of shipment.

Analysis Note

Routinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
16157PROTGARO16266
Protein G Agarose/Salmon Sperm DNA, 2.5 mL for use in chromatin immunoprecipitations (ChIP assays)

Sigma-Aldrich

16201

Protein G Agarose/Salmon Sperm DNA, 2.5 mL

Protein A Agarose/Salmon Sperm DNA, 2.5 mL for use in chromatin immunoprecipitations (ChIP assays)

Sigma-Aldrich

16157

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

Protein G Agarose >98% (HPLC and SDS-PAGE), suspension

Roche

PROTGARO

Protein G Agarose

Protein G Agarose, Fast Flow Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.

Millipore

16266

Protein G Agarose, Fast Flow

description

for use in chromatin immunoprecipitations (ChIP assays)

description

for use in chromatin immunoprecipitations (ChIP assays)

description

>98% (HPLC and SDS-PAGE), suspension

description

Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.

technique(s)

ChIP: suitable

technique(s)

ChIP: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable

form

liquid

form

liquid

form

suspension

form

liquid

suitability

suitable for immunoprecipitation

suitability

suitable for immunoprecipitation

suitability

-

suitability

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

-

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Roche

manufacturer/tradename

Upstate®

Storage Class

10 - Combustible liquids

wgk

WGK 1

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