487664
p-Nitrophenyl Phosphate Substrate Buffer
This p-Nitrophenyl Phosphate Substrate Buffer is validated for use in ELISA.
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| Size/SKU | Availability | Price |
|---|---|---|
500 mL | Estimated to ship TODAY | $221.00 |
About This Item
Quality Segment
sterility
non-sterile
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
dilution
(ELISA )
color
clearlight yellow
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Alkaline phosphatase substrate buffer
Other Notes
Suggested procedure for use of p-NPP in alkaline phosphatase-based ELISAs:
1. To 39 parts distilled water add 1 part 50X p-NPP Concentrate (Cat. No. 487663) and 10 parts p-NPP Substrate Buffer. Mix well and store protected from light. This diluted reagent is suitable for up to 10 h after preparation.
2. Complete all required incubations with antibodies and alkaline phosphatase-labeled probes.
3. Wash plate wells at least 4 times with Tris-buffered saline (TBS) containing 0.1% Tween®-20 detergent. DO NOT USE PHOSPHATE BUFFERS; inorganic phosphate is a potent inhibitor of alkaline phosphatase.
4. After the final wash, shake and blot all residual buffer from the wells.
5. Add 100 µl of 1X p-NPP Substrate Solution to each well and incubate for 15-60 min. (If a kinetic study is desired, readings at 405 nm may be taken at intermediate intervals).
[Note: Optimal incubation times may vary depending on the amount of enzyme present. If color develops too quickly, dilution of the probe, antibody, or alkaline phophatase-labeled reagent may be required.]
6. Stop the reaction by adding 100 µl of 1 N NaOH.
7. Read absorbance at 405 nm.
Note: Variations in time, reagent volumes, and temperature may require further standardization by the user.
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| description This p-Nitrophenyl Phosphate Substrate Buffer is validated for use in ELISA. | description p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM). | description Excellent substrate for alkaline phosphatase-based ELISA assays. | description Excellent substrate for alkaline and acid phosphatase-based ELISA assays. |
| form liquid | form crystalline solid | form solid | form - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition OK to freeze, protect from light | storage condition OK to freeze, protect from light | storage condition OK to freeze | storage condition OK to freeze |
| shipped in wet ice | shipped in ambient | shipped in ambient | shipped in ambient |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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