Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

5.31085

MLS000544460

≥97% (HPLC), Eya2 Phosphatase Inhibitor, solid

Synonym(s):

Eya2 Phosphatase Inhibitor, MLS000544460, 3-Fluoro-N-(( E)-(5-pyridin-2-ylsulfanylfuran-2-yl)methylideneamino)benzamide, ( E)-3-Fluoro-Nʹ-((5-(pyridin-2-ylthio)furan-2-yl)methylene)benzohydrazide, Eab1 Inhibitor, Eyes Absent Homolog 2 Inhibitor

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
25 mg
Check Cart for Availability
$224.00

About This Item

Empirical Formula (Hill Notation):
C17H12FN3O2S
CAS Number:
352336-36-8
Molecular Weight:
341.36
MDL number:
MFCD28064079
UNSPSC Code:
12352200
NACRES:
NA.77

$224.00

Check Cart for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Eya2 Phosphatase Inhibitor, MLS000544460,

assay

≥97% (HPLC)

Quality Segment

100

form

solid

potency

2.0 μM Ki

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

beige

solubility

DMSO: 100 mg/mL

storage temp.

2-8°C

General description

A cell-permeable furylidenebenzohydrazide compound that acts as a reversible inhibitor against Eya2 C-terminal Eya domain (ED) protein tyrosine phosphatase (PTP) activity (IC50 = 4.1 µM; 100 nM ED & 5 µM Mg2+; Substrate = 25 µM OMFP) via affinity docking at an allosteric pocket located opposite to the active site (KD = 6.1 or 2.0 µM, respectively, with or without 5 mM exogenous Mg2+; KD = 0.8 µM with 10 mM EDTA and no exogenous Mg2+), while being ineffective against the PTP activity of Eya3 & PTP1B or the Ser/Thr phosphatase activity of PPM1A & Scp1. Shown to reduce Eya2 overexpression-induced migration enhancement in MCF10A cultures (17% vs. 54% enhancement with or without 10 µM inhibitor). The inhibition appears to be non-competitive with respect to substrate in the presence of 5 mM Mg2+, while mixed mode of inhibition is observed at a low Mg2+ concentration of 5 µM.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Eya2 C-terminal Eya domain (ED) protein tyrosine phosphatase
Reversible: yes

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Krueger, A.B., et al. 2014. J. Biol. Chem.289, 16349.

Krueger, A.B., et al. 2013. J. Biomol. Screen.18, 85.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
540218M555460M504907
Eya2 Phosphatase Inhibitor, MLS000544460

Sigma-Aldrich

531085

Eya2 Phosphatase Inhibitor, MLS000544460

LYP Inhibitor II, LTV-1 The LYP Inhibitor II, LTV-1 controls the biological activity of LYP. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Sigma-Aldrich

540218M

LYP Inhibitor II, LTV-1

Rho Inhibitor, Rhosin Rho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.

Millipore

555460M

Rho Inhibitor, Rhosin

Tankyrase 1/2 Inhibitor VI, G007-LK

Sigma-Aldrich

504907

Tankyrase 1/2 Inhibitor VI, G007-LK

description

-

description

The LYP Inhibitor II, LTV-1 controls the biological activity of LYP. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

description

Rho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.

description

-

assay

≥97% (HPLC)

assay

≥98% (HPLC)

assay

≥93% (HPLC)

assay

≥98% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

solid

form

powder

form

powder

form

powder

solubility

DMSO: 100 mg/mL

solubility

DMSO: 50 mg/mL

solubility

DMSO: 10 mg/mL

solubility

DMSO: 25 mg/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, protect from light

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters