The recommended volume per gel is 5ul. The package volume is 500ul, thus it is sufficient for 100 lanes or gels. The product is packaged in a 25ml bottle.
69079
Perfect Protein Markers, 10-225 kDa
Synonyme(s) :
protein markers, protein molecular weight markers, protein standards
pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| À vous/Référence | Disponibilité | Prix |
|---|---|---|
100 assays | Vérifier la disponibilité des articles du panier | 151,00 € |
A propos de cet article
NACRES:
NC.01
UNSPSC Code:
41105337
151,00 €
Vérifier la disponibilité des articles du panier
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderform
liquid
Quality Segment
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
General description
The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
Features and Benefits
• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic (F)
1 of 1
Cet article | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form solid |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 100 |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition - |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
-
Hi, Can you please let know the packaging volume and the container that the product will be supplied? The packaging details is a bit confusing, as it states 25ml and 100 lane. And also please provide the details of how many microliters to be added to gel?
1 réponse-
Utile ?
-
-
Hello, Please; I used your product Perfect Protein™ Markers, 10-225 kDa, in my Lab for SDS. However, I do not know the band range interpretation. Please, I need assistance with the band range interpretation. I will appreciate any document showing such.
1 réponse-
As mentioned on the product detail page, under the "Description" section, the Perfect Protein Markers, 10-225 kDa, include the protein sizes of 10, 15, 25, 35, 50, 75,100,150, and 225kDa . Please refer to page 72 of the brochure linked on the product detail page to see the image of the 10-225 kDa markers.
Utile ?
-