The recommended volume per gel is 5ul. The package volume is 500ul, thus it is sufficient for 100 lanes or gels. The product is packaged in a 25ml bottle.
69079
Perfect Protein Markers, 10-225 kDa
Synonym(s):
protein markers, protein molecular weight markers, protein standards
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 assays | Estimated to ship TODAY | $171.00 |
About This Item
NACRES:
NC.01
UNSPSC Code:
41105337
$171.00
Estimated to ship TODAYDetails
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
General description
The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
Features and Benefits
• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic (F)
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form solid |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 100 |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename - |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Csenge Filep et al.
Analytical chemistry, 92(5), 4023-4028 (2020-02-06)
The temperature-dependent migration of molecular weight protein size standards and several biotherapeutic proteins were studied in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) in the interval from 15 to 60 °C using borate cross-linked dextran sieving matrix. Arrhenius plots were
Rebeccah A Warmack et al.
The Journal of biological chemistry, 294(32), 12203-12219 (2019-06-27)
Transparency in the lens is accomplished by the dense packing and short-range order interactions of the crystallin proteins in fiber cells lacking organelles. These features are accompanied by a lack of protein turnover, leaving lens proteins susceptible to a number
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 69079-3 | 07790788052515 |
| 76740-25ML | 04055977265798 |
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Hi, Can you please let know the packaging volume and the container that the product will be supplied? The packaging details is a bit confusing, as it states 25ml and 100 lane. And also please provide the details of how many microliters to be added to gel?
1 answer-
Helpful?
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Hello, Please; I used your product Perfect Protein™ Markers, 10-225 kDa, in my Lab for SDS. However, I do not know the band range interpretation. Please, I need assistance with the band range interpretation. I will appreciate any document showing such.
1 answer-
As mentioned on the product detail page, under the "Description" section, the Perfect Protein Markers, 10-225 kDa, include the protein sizes of 10, 15, 25, 35, 50, 75,100,150, and 225kDa . Please refer to page 72 of the brochure linked on the product detail page to see the image of the 10-225 kDa markers.
Helpful?
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