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EZHS100B

Human S100B ELISA Kit

quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma

Synonym(s):

Protein S100B, S-100 protein beta chain, S100 calcium-binding protein B

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Pack SizeSKUAvailabilityPrice
96 wells
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$898.00

About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Input:
sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice

$898.00

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Product Name

Human S100B ELISA, EZHS100B-33K, This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

P04271

application(s)

research use

assay range

accuracy: 93%
(CSF), accuracy: 94%
(serum), sensitivity: 2.7 pg/mL, standard curve range: 2.7-2000 pg/mL, inter-assay cv: <4%
intra-assay cv: <4%

detection method

colorimetric (450nm)

shipped in

wet ice

storage temp.

2-8°C

General description

EMD Millipore’s Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum and plasma. This kit has not been tested for cell culture supernatants.
S100B is a calcium-binding protein produced mainly by astrocytes in the central nervous system and has been implicated in the development and maintenance of the nervous system. Although the mechanism of S100B secretion is unknown, it appears to be affected by oxidative stress. At nanomolar concentrations (normal levels), S100B is able to protect neurons against glutamate toxicity. Glial damage or astrocytic reactions to neural injury (reactive astrogliosis) causes an increase in S100B to micromolar concentrations. High levels of S100B will further increase neuronal damage. High levels of S100B are considered a biomarker that could indicate damage or dysfunction of CNS.

Application

Research Category
Neuroscience
This is a same day assay
Used to detect/quantify: S100B

Biochem/physiol Actions

The antibody pair used in this assay measures Human S100B and has no cross-reactivity with S100A1, S100A6 and S100A13.

This assay detects S100B in multiple species, including: bovine, hamster, horse, monkey, mouse, pig, rabbit, and rat.

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

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This Item
ABN59S2532SAB5700647
Human S100B ELISA, EZHS100B-33K This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum &amp; plasma.

Millipore

EZHS100B

Human S100B ELISA, EZHS100B-33K

Anti-S100B Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN59

Anti-S100B Antibody

Monoclonal Anti-S-100 (&#946;-Subunit) antibody produced in mouse clone SH-B1, ascites fluid

Sigma-Aldrich

S2532

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse

Anti-S100B antibody produced in rabbit

Sigma-Aldrich

SAB5700647

Anti-S100B antibody produced in rabbit

species reactivity

human

species reactivity

human

species reactivity

feline, pig, bovine, sheep, goat, rabbit, canine, human, rat

species reactivity

rat, mouse, human

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

input

-

input

-

input

-

assay range

accuracy: 93%
(CSF), sensitivity: 2.7 pg/mL, inter-assay cv: <4%
intra-assay cv: <4%, accuracy: 94%
(serum), standard curve range: 2.7-2000 pg/mL

assay range

-

assay range

-

assay range

-

Quality Level

200

Quality Level

100, 300

Quality Level

200

Quality Level

100

detection method

colorimetric (450nm)

detection method

-

detection method

-

detection method

-

UniProt accession no.

P04271

UniProt accession no.

P04271

UniProt accession no.

P04271

UniProt accession no.

P04271

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Certificates of Analysis (COA)

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Alison Crichton et al.
Journal of neurotrauma, 38(8), 1151-1163 (2019-12-28)
Despite many children experiencing fatigue after childhood brain injury, little is known about the predictors of this complaint. To date, traditional indices of traumatic brain injury (TBI) severity have not predicted reliably persisting fatigue (up to three years post-injury). This

Global Trade Item Number

SKUGTIN
EZHS100B-33K04053252417313

Questions

1–2 of 2 Questions  

  1. Is the EZHS100B-33K ELISA assay compatible with human saliva samples?

    1 answer

    1. The kits have not been validated for use with saliva; however, some guidelines are available for processing saliva samples:


      1. Add protease inhibitor cocktail (Product P2714) at a 1:500 ratio to the saliva.
      2. Centrifuge the mixture at 10,000 rpm for 10 minutes.
      3. Dilute the supernatant in a 1:2 ratio with Assay Buffer before to setting up the assay.
      4. Run assay with Assay Buffer as the matrix for the standard curve.
      5. Multiply by 2 for the final result.


      It should be noted that there are no references available for the S100B ELISA kit being used with saliva samples.

      Helpful?

  2. Which antibodies have been used for the Human S100B ELISA kit (EZHS100B-33K)?

    1 answer

    1. The origins of the antibodies utilized in the kit are considered proprietary. Nevertheless, some information about the antibodies can be shared. The capture antibody is a monoclonal mouse anti-S100B, with bovine brain S100B used as the immunogen. The detection antibody is a polyclonal rabbit anti-S100, and the immunogen for this is also sourced from bovine brain S100.

      Helpful?

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