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Anti-ZO-1 Antibody, clone 5G6.1

Name: Anti-ZO-1 Antibody, clone 5G6.1
Brand: MM
Price: 477 USD
Availability: InStock

clone 5G6.1, from mouse

Synonym(s):

Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, ZO-1

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Size/SKU	Availability	Price
100 μg	Estimated to ship onOctober 28, 2026	$477.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

5G6.1, monoclonal

Application:

IHC, WB

Citations:

$477.00

Estimated to ship onOctober 28, 2026Details

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Properties

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5G6.1, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_003248

UniProt accession no.

Q07157

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TJP1(7082)

Description

General description

Tight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.

~195 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human ZO-1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ZMS1539	AB2272	HPA001637
Sigma-Aldrich MABT339 Anti-ZO-1 Antibody, clone 5G6.1	ZMS1539 Anti-ZO-1 Antibody, clone 5G6.1 ZooMAb^® Mouse Monoclonal	Sigma-Aldrich AB2272 Anti-ZO-1 Antibody	Sigma-Aldrich HPA001637 Anti-TJP1 antibody produced in rabbit
description clone 5G6.1, from mouse	description recombinant, expressed in HEK 293 cells	description from rabbit, purified by affinity chromatography	description Ab2, Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
antibody form purified immunoglobulin	antibody form purified antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Gene Information human ... TJP1(7082)	Gene Information human ... TJP1(7082)	Gene Information human ... TJP1(7082)	Gene Information human ... TJP1(7082)
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
clone 5G6.1, monoclonal	clone 5G6.1, recombinant monoclonal	clone polyclonal	clone polyclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity rat, human	species reactivity human, rat	species reactivity human	species reactivity human

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

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