The complete formulation of this product is considered is proprietary and may not be shared. This solution contains a chelating agent and a stabilizer to maintain cell surface activity. Should a specific formulation be required, please refer to product E8008, a 0.02% EDTA solution in DPBS, which is also suitable for non-enzymatic cell dissociation.
S-014-M
Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml
The Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.
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Synonym(s):
Cell Dissociation Solution
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Check Cart for Availability | $26.60 |
500 mL | Check Cart for Availability | $65.10 |
About This Item
UNSPSC Code:
12352204
NACRES:
NA.75
eCl@ss:
32160801
Quality Segment
form
liquid
manufacturer/tradename
Specialty Media
input
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type cardiac stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
General description
Enzyme-Free Cell Dissociation Solution is a PBS based proprietary formulation of chelating agents and agents to stabilize their activity on cells. The enzymatic activity of this formulation is comparable to trypsin. It preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.
Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
Preparation Note
Product can be stored at room temperature or at 4°C. Shelf life is 2 years
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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本製品Enzyme Free Cell Dissociation Solution PBS Based (1X), liquidを用いて機能アッセイをしたいのですが、成分を開示していただけますでしょうか。
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