SCC447
OSUMMER.3 Mouse NRAS-Mutant Melanoma Cell Line
Human
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human
Growth mode:
N/A
Manufacturer/tradename:
Millipore
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenProduktname
OSUMMER.3 Mouse NRAS-Mutant Melanoma Cell Line,
biological source
human
Quality Segment
packaging
vial of ≥1X10⁶ cells/vial mg
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
cell analysis: suitable
shipped in
liquid nitrogen
storage temp.
−196°C
Application
Each vial contains >1X106 viable cells.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
The OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation) cell lines fill a previously unfilled gap in melanoma biology. The OSUMMER cell lines are syngeneic to C57BL/6 laboratory mice and have genetic profiles that are similar to human tumors, making them responsive to immunotherapy treatments. These NRAS-Mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-25% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model.
OSUMMER.3 NRAS mouse melanoma cell line was derived from a female TN mouse exposed to UVA and UVB radiation. OSUMMER.3 carries the specific NRAS mutation Q61R and can form tumors in vivo without Matrigel injection. WES revealed high SNV (Single Nucleotide variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations. OSUMMER.3 cells express the melanoma markers, PMEL and MelanA, and exhibit robust growth in culture.
Source
OSUMMER.3 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (1).
References
1. Bowman, R. L., et al. (2021). UVB mutagenesis differs in Nras-and Braf-mutant mouse models of melanoma. Life Science Alliance, 4(9).
OSUMMER.3 NRAS mouse melanoma cell line was derived from a female TN mouse exposed to UVA and UVB radiation. OSUMMER.3 carries the specific NRAS mutation Q61R and can form tumors in vivo without Matrigel injection. WES revealed high SNV (Single Nucleotide variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations. OSUMMER.3 cells express the melanoma markers, PMEL and MelanA, and exhibit robust growth in culture.
Source
OSUMMER.3 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (1).
References
1. Bowman, R. L., et al. (2021). UVB mutagenesis differs in Nras-and Braf-mutant mouse models of melanoma. Life Science Alliance, 4(9).
Features and Benefits
OSUMMER.3 carries the specific NRAS mutation Q61R and may be used to create syngeneic mouse melanoma models.
Preparation Note
The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| growth mode N/A | growth mode N/A | growth mode N/A | growth mode N/A |
| technique(s) cell analysis: suitable | technique(s) cell analysis: suitable | technique(s) cell analysis: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable |
| biological source human | biological source human | biological source - | biological source human |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in liquid nitrogen |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Verwandter Inhalt
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC447 | 04065267034621 |