SCC468
T24T Human Bladder Carcinoma Cell Line
Human
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| Size/SKU | Availability | Price |
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1 vial | Check Cart for Availability | $1,130.00 |
About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human
Growth mode:
N/A
Manufacturer/tradename:
Millipore
$1,130.00
Check Cart for Availability
Product Name
T24T Human Bladder Carcinoma Cell Line,
biological source
human
Quality Segment
packaging
vial of ≥1X10⁶ cells/vial mg
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
cell analysis: suitable
shipped in
liquid nitrogen
storage temp.
−196°C
Application
Bladder cancer originates from the epithelium that covers the inner surface of the bladder. The most common type is urothelial or transitional carcinoma (1). Most of the newly diagnosed cases (75%) are non-invasive, if the tumor becomes invasive it is more likely to metastasize to lymph nodes and other organs. Two common alterations have been identified in non-invasive tumors, deletion of chromosome 9 and a point mutation in FGFR3 (Fibroblast growth factor receptor 3) (2). Activating mutations in RAS gene family members are also common in bladder carcinoma and it is estimated that activation of the RAS-MAPK pathway may contribute to over 80% of the non-invasive cases (1). Loss of function of tumor suppressor genes like TP53 and RB1 are common in cases of invasive disease (1). After diagnosis the patient requires resection of the tumor and in cases of invasive disease chemotherapy is also provided.The original cell line T24 has been used to study bladder tumor biology but has some limitations because it lacks tumorigenicity when injected into immunocompromised mice. In contrast, T24T has increased metastatic ability when tested in vivo and expresses higher levels of HRAS mRNA (3). Increased HRAS activity leads to focal adhesion disassembly and los of CDH1 and b-catenin from intercellular junctions (3). Contact inhibition growth is reduced in T24T compared with T24 (3). Additionally, T24T does not inhibit its motile activity at higher density like T24 does.T24 cell line was generated from an invasive human transitional cell carcinoma of the bladder (4). T24T is a derivative of T24.1. Nat Rev Dis Primers 2017, 3: 17022.2. Int J Cancer 2006, 119(5): 1212-1215.3. Genes Chromosomes Cancer 2000, 27(3): 252-263.4. Int J Cancer 1973, 11(3): 765-773.
Each vial contains ≥1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.
Features and Benefits
T24T human bladder cancer cell line is a useful model for the study of metastatic bladder cancer.
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human | biological source human | biological source mouse | biological source human |
| growth mode N/A | growth mode - | growth mode - | growth mode N/A |
| technique(s) cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in dry ice |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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