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SCT036

BioTracker 625 Red GSH Dye

Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.

Synonym(s):

Live cell imaging probe

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Size/SKUAvailabilityPrice
2 x 25 nmol
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$553.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.47
eCl@ss:
32161000

$553.00


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technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

storage temp.

-10 to -25°C

General description

Glutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals. Alterations in glutathione homeostasis are associated with a variety of diseases; therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes.

The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.

Spectral Properties
Absorbance: 520nm
Emission: 625nm and 582nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Preparation Note

Store BioTracker 625 Red GSH Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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description

Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.

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description

Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels.

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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