The dye does not substantially affect cell viability and is considered non-toxic. Determining the optimal dye incubation time for specific cells is necessary. Please see the staining protocol on the website under View all Documentation-> More Documents-> Data sheets. The dye primarily localizes to cell surface membranes shortly after staining. In live cells, the stained membranes become internalized by endocytosis, resulting in primarily intracellular staining over the course of a few hours in culture.
SCT108
BioTracker 655 Red Cytoplasmic Membrane Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Synonym(s):
Live cell imaging probe, Live cell membrane imaging probe
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Estimated to ship TODAY | $373.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
$373.00
Estimated to ship TODAYDetails
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
2-8°C
General description
Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 644nm
Emission: 665nm
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 644nm
Emission: 665nm
Application
Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Liquid
Preparation Note
Store BioTracker 655 Red Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 644nm
Emission: 665nm
Absorbance: 644nm
Emission: 665nm
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| description Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies. | description Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies. | description Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies. | description Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. - |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
55.4 °F
flash_point_c
13 °C
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Can the dye stay in the culture media after addition or does the media need to be exchanged? Do you have any indication of how viability is affected when the dye remains in the media vs when media is exchanged?
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Helpful?
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Can this product be used to bind to cell membrane of human smooth muscle cells?
1 answer-
This product is a lipophilic carbocyanine membrane dye, it is not species-specific. This compound is not tested specifically with human smooth muscle, however, there is no reason to suspect that this material would not be suitable.
Please see the links below to review additional publications that may be helpful:
Histochemistry 1992 May;97(4):329-33. doi: 10.1007/BF00270034. PMID: 1618647 https://pubmed.ncbi.nlm.nih.gov/1618647/
ACS Omega 2020, 5, 20, 11829–11835 https://doi.org/10.1021/acsomega.0c01416 https://pubs.acs.org/doi/10.1021/acsomega.0c01416Please see the link below to review additional product information on the BioTracker Live Cell Stains and Dyes:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/organelle-dyes-and-stainsHelpful?
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