Below is a link to the product bulletin, which contains the assay protocol.
SCT127
BioTracker 640 Red C2(FM4-64) Synaptic Dye
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Synonym(s):
Live cell imaging probe
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Check Cart for Availability | $470.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
$470.00
Check Cart for Availability
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
General description
BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Application
Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Lyophilized
Preparation Note
Store BioTracker 640 Red C2(FM4-64) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.
Analysis Note
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| description Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses. | description Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses. | description Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses. | description Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in ambient | shipped in ambient | shipped in ambient |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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BioTracker 640 Red C2（FM4-64）シナプス色素でシナプス小胞を染色する操作手順を教えていただきたいです。
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