Skip to Content
MilliporeSigma

Skip To

WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
2 x 25 mL

Estimated to ship TODAY

$91.90
2 x 50 mL

Estimated to ship TODAY

$166.00
2 x 250 mL

Estimated to ship TODAY

$501.00

About This Item

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053

$91.90


Estimated to ship TODAYDetails


​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


conjugate

peroxidase conjugate

Quality Segment

packaging

pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution

manufacturer/tradename

Immobilon®

technique(s)

immunodetection: suitable, western blot: suitable

membrane area

5000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Immobilon Western HRP Substrate is compatible with all commonly used buffers and blocking reagents and provides high sensitivity for chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes.

Application

Immobilon Western Chemiluminescent HRP Substrate is suitable for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
WBLUCWBULS0100WBLUR
description

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

description

Western blotting, dot/slot blotting

description

Western blotting, HRP

description

Western blotting, dot/slot blotting

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

-

conjugate

peroxidase conjugate

manufacturer/tradename

Immobilon®

manufacturer/tradename

Luminata

manufacturer/tradename

-

manufacturer/tradename

Luminata


Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

WGK

WGK 2



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library




Questions

  1. What is hydrogen peroxide concentration in Immobilon Western Chemiluminescent HRP Substrate, WBKLS0500?

    1 answer
    1. The concentration of the Hydrogen Peroxide component is considered proprietary, but has been optimized for use with the Luminol Reagent. Equal volumes of each component are required, as indicted in the table below.

      Please see the link below to review additional information available in the product user guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/865/668/p36599.pdf

      Helpful?

Reviews

No rating value

Active Filters