Key Documents

View All Documentation

β-Galactose Dehydrogenase

Name: β-Galactose Dehydrogenase
Brand: ROCHE
Price: 95.8 USD
Availability: InStock

from Pseudomonas fluorescens

Synonym(s):

D-galactose dehydrogenase, D-galactose:NAD+ 1-oxidoreductase

Select a Size

Change View

Size/SKU	Availability	Price
1 mg	Available to ship TODAYfromMILWAUKEE	$95.80
5 mg	Check Cart for Availability	$286.00

About This Item

UNSPSC Code:

12352204

EC Number:

1.1.1.48 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)

Biological source:

Pseudomonas fluorescens

Recombinant:

expressed in E. coli

$95.80

Available to ship TODAYDetails

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Pseudomonas fluorescens

recombinant

expressed in E. coli

form

suspension

specific activity

~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)

mol wt

64,000 Da

packaging

pkg of 1 mL (10104973001 [1 mg]), pkg of 1 mL (10104981001 [5 mg])

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

white

optimum pH

8.4 (0.1 M in Tris buffer)

pH range

4.6-9.2

solubility

water: soluble

suitability

suitable for enzyme test

UniProt accession no.

(T1WEA6 · T1WEA6_PSEFL)

foreign activity

ADH <0.01%, LDH <0.5%, NADH oxidase <0.1%, galactosidase <0.01%

storage temp.

2-8°C

Description

General description

β-Galactose Dehydrogenase plays a crucial role in galactose metabolism by catalyzing the pyridine-nucleotide-dependent dehydrogenation of β-D-galactopyranose to yield NADH and D-galactono-l,5-1actone. D-galactose dehydrogenase from P. fluorescens is a member of the oxidoreductases family. It is composed of two identical subunits and has a molecular weight of 64,000. Additionally, the enzyme contains two binding sites.

Application

β-Galactose Dehydrogenase has been used as a reporter enzyme in neuraminidase (NA) kinetic parameters for the natural substrates 6′-Sialyl-N-acetyllactosamine (6SLN) and 3′-Sialyl-N-acetyllactosamine (3SLN).

Biochem/physiol Actions

Galactose dehydrogenase will oxidize D-galactose [K_m = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (K_m = 0.24 mM; relative rate = 1.0) or NADP (K_m = 2.3 mM; relative rate = 0.32).

Physical form

Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

Analysis Note

Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH

Other Notes

For life science research only. Not for use in diagnostic procedures.

Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).

One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	10662046001	FORMD-RO	G7907
Roche BGALD-RO β-Galactose Dehydrogenase	Roche 10662046001 β-Galactose Dehydrogenase S	Roche FORMD-RO Formate Dehydrogenase	Sigma-Aldrich G7907 Galactose Oxidase from Dactylium dendroides
description from Pseudomonas fluorescens	description suspension, ~80 units/mg protein (At 25 °C with galactose as the substrate.), pkg of 50 U	description from Candida boidinii	description ≥30 units/mg solid
technique(s) activity assay: suitable	technique(s) -	technique(s) activity assay: suitable	technique(s) -
biological source Pseudomonas fluorescens	biological source -	biological source yeast (Candida boidini)	biological source fungus (Dactylium dendroides)
specific activity ~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)	specific activity 80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)	specific activity ~0.4 units/mg protein (At 25 °C with formate as the substrate.)	specific activity ≥30 units/mg solid
form suspension	form suspension	form lyophilized	form lyophilized
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -
suitability suitable for enzyme test	suitability -	suitability suitable for gel electrophoresis	suitability -

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c