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NADP-RO

Roche

NADP

Disodium salt

Synonym(s):

β-Nicotinamide adenine dinucleotide phosphate disodium salt, β-NADH phosphate disodium salt, β-NADP-Na2, Triphosphopyridine nucleotide disodium salt

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100 mg
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RM 521.00
500 mg
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RM 1,868.00
1 g
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RM 2,791.00
5 g
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RM 11,725.00

About This Item

Empirical Formula (Hill Notation):
C21H26N7Na2O17P3
CAS Number:
24292-60-2
Molecular Weight:
787.37
UNSPSC Code:
12352302
PubChem Substance ID:
329818702
MDL number:
MFCD00065390
NACRES:
NA.54
Assay:
≥97%
Form:
powder
Storage temp.:
2-8°C

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assay

≥97%

form

powder

mol wt

Mr 743.4, Mr 787.4

packaging

pkg of 1 g (10128058001), pkg of 100 mg (10128031001), pkg of 5 g (10240354001), pkg of 500 mg (10128040001)

manufacturer/tradename

Roche

pH

4.0 ± 1.0(10mg/mL)

λmax

260 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

[Na+].[Na+].NC(=O)c1ccc[n+](c1)C2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP(O)([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O

InChI

1S/C21H28N7O17P3.2Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39);;/q;2*+1/p-2/t10-,11-,13-,14-,15-,16-,20?,21-;;/m1../s1

InChI key

WSDDJLMGYRLUKR-FHQWUTKFSA-L

General description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair.[1] The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.[2]

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen.[3] It has also been used for the hexokinase assay.[4]

Preparation Note

Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C

Analysis Note

Absorbance coefficients: 18.0 x 103 [l x mol-1 x cm-1] at 260 nm, 5.9 x 103 [l x mol-1 x cm-1] at 333 nm (CN complex)

Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A340 approximately 0.100.

Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A360 approximately 0.200-0.250.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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NADP Disodium salt

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NADP, Monosodium Salt A coenzyme that is widely distributed in living matter. Participates in oxidation-reduction reactions. Spectra (pH 7.0): 250 nm/260 nm: 0.78-0.88; 280 nm/260 nm: 0.16-0.26.

Millipore

481971-M

NADP, Monosodium Salt

NAD Grade I, free acid

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NAD Grade II, free acid

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NAD

manufacturer/tradename

Roche

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Roche

manufacturer/tradename

Roche

form

powder

form

solid

form

lyophilized

form

lyophilized

assay

≥97%

assay

≥92% (enzymatic), ≥95% (UV)

assay

100% (dry weight)

assay

98% (dry weight)

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

packaging

pkg of 1 g (10128058001), pkg of 5 g (10240354001), pkg of 100 mg (10128031001), pkg of 500 mg (10128040001)

packaging

-

packaging

pkg of 1 g (10127965001), pkg of 5 g (10127973001)

packaging

pkg of 1 g (10127981001), pkg of 10 g (10621650001), pkg of 5 g (10127990001)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
92551721
83976135
81428121
79416655
74881551

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Malgorzata Adamczyk et al.
Methods in enzymology, 500, 233-257 (2011-09-29)
In vitro enzymatic assays of cell-free extracts offer an opportunity to assess in vivo enzyme concentrations. If performed under conditions that resemble the conditions in vivo, they may also reveal some of the capacities and properties of the same enzymes
Redox modulation of glucose-6-P dehydrogenase in Anacystis nidulans and its 'uncoupling' by phage infection.
C Cséke et al.
FEBS letters, 126(1), 85-88 (1981-04-06)
Jinrui Zhang et al.
Scientific reports, 5, 12846-12846 (2015-08-06)
Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofactors. NADP(H) are critical components
View All Related Papers

Global Trade Item Number

SKUGTIN
1012805800104061838250377
1024035400104061838250506
1012804000104061838667632
1012803100104061838667625