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21819

Carbol-Fuchsin solution according to Kinyoun

suitable for microscopy

Synonym(s):

Kinyoun’s carbol-Fuchsin

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Size/SKUAvailabilityPrice
50 mL
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$111.00
250 mL
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$480.00

About This Item

NACRES:
MA.02
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:

$111.00


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form

liquid

Quality Segment

composition

fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)

technique(s)

microbe id | staining: suitable

impurities

phenol

color

red to very dark red

density

0.99 g/mL at 20 °C

ε (extinction coefficient)

77 at 546 nm in 50% ethanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]

InChI

1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3

InChI key

IMOKRVRFBCWWGH-UHFFFAOYSA-N

General description

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

Application

  • Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
  • It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
  • It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

Biochem/physiol Actions

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

Other Notes

Stain used for the demonstration of tuberculosis bacteria

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This Item
21820F812987794
description

suitable for microscopy

description

suitable for microscopy

description

used in tissue staining

description

suitable for microscopy

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy

suitability

bacteria, suitable for microscopy

suitability

-

suitability

suitable for microscopy

color

red to very dark red

color

-

color

blue-green

color

-

form

liquid

form

liquid

form

powder

form

liquid

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100


signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

109.9 °F - closed cup

flash_point_c

43.3 °C - closed cup



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