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Carbol-Fuchsin solution according to Ziehl-Neelsen

Name: Carbol-Fuchsin solution according to Ziehl-Neelsen
Brand: SIAL
Price: 61.7 USD
Availability: InStock

suitable for microscopy

Synonym(s):

Carbol fuchsin solution

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Size/SKU	Availability	Price
100 mL	Available to ship TODAYfromMILWAUKEE	$61.70
1 L	Available to ship TODAYfromMILWAUKEE	$136.00

About This Item

NACRES:

MA.02

PubChem Substance ID:

329752210

UNSPSC Code:

12171500

MDL number:

MFCD00013129

$61.70

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Properties

Quality Segment

100

form

liquid

technique(s)

microbe id | staining: suitable

impurities

phenol

density

0.99 g/mL at 20 °C

ε_max

9.36 at 542-552 nm in 50% ethanol

suitability

bacteria, suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

storage temp.

room temp

SMILES string

Cl[H].Cc1cc(ccc1N)\C(=C2\C=CC(=N)C=C2)c3ccc(N)c(C)c3

InChI

1S/C21H21N3.ClH/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17;/h3-12,22H,23-24H2,1-2H3;1H

InChI key

YCIVZWKFFBDLTG-UHFFFAOYSA-N

Description

General description

Ziehl-Neelsen staining method using Carbol-Fuchsin is a crucial diagnostic tool used for the detection of acid-fast mycobacteria in tissue sections.

Application

Carbol-Fuchsin solution according to Ziehl-Neelsen is suitable for use in staining assays for acid-resistant, rod-shaped bacteria.

Other Notes

Stain for the assay of acid-resistant rod-shaped bacteria

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1 of 1

This Item	87794	21819	215597
Sigma-Aldrich 21820 Carbol-Fuchsin solution according to Ziehl-Neelsen	Sigma-Aldrich 87794 Gram′s fuchsin solution	Sigma-Aldrich 21819 Carbol-Fuchsin solution according to Kinyoun	Sigma-Aldrich 215597 Basic Fuchsin
description suitable for microscopy	description suitable for microscopy	description suitable for microscopy	description Dye content >85 %
suitability bacteria, suitable for microscopy	suitability suitable for microscopy	suitability suitable for microscopy	suitability -
technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable
form liquid	form liquid	form liquid	form solid
application(s) diagnostic assay manufacturing hematology histology microbiology	application(s) diagnostic assay manufacturing hematology histology microbiology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100

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pictograms

GHS08,GHS05

signalword

Danger

hcodes

H314,H341,H412

pcodes

P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Questions

7 months ago

I bought Carbol-Fuchsin solution according to Ziehl-Neelsen (SKU 21820-1L) from Merck for detection of mycobacterium on tissue sections. No bacteria detected, even in the positive control slides. Is this the right reagent? Can you recommend me a protocol?

1 answer
1. Technical Support
  
  5 months ago
  
  Carbol-Fuchsin solution according to Ziehl-Neelsen, catalog number 21820, should stain acid fast mycobacterium. Quality Control testing performed for product 21820 does not include staining tissue sections. The procedure recommended by the manufacturing site is for smears. The recommend procedure can be found in Staining Procedures: Fourth Edition by George Clark, Williams & Williams, Baltimore 1981 page 380-381.
  Products are guaranteed 1 year from the date of shipping. If no staining is observed, please contact your local Technical Support team to file a formal complaint.
  There are other Carbol Fuchsin solutions that are performance tested during quality testing to ensure acid fast bacillus organisms are demonstrated on tissue sections. Tissue Control slides are also sold as catalog number TTR001-25ea. Catalog numbers for the Carbol Fuchsin are HT8018-250ml or HT80116-500ml. The product numbers for the counterstain for the carbol fuchsin solution are HT8028-250ml or HT80216-500ml. Instructions For Use are found on the website for the Carbol Fuchsin solutions and the Malachite Green counterstain.
  
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