This product is not listed as sterile and is not specifically qualified for cell culture or mouse/in vivo models. Suitability for such applications must be validated by the customer. The suspension contains approximately 1.8 × 10^8 particles/mL based on 10% solids, density 1.05 g/mL, and a 10 µm particle size. Should a sterile preparation be required, consider pasteurization instead of autoclaving. Pasteurization involves heating a suspension of the beads for 24 hours at 70-80 degrees C. Polystyrene will soften at autoclave temperatures and then aggregate upon cooling.
72986
Micro particles based on polystyrene
size: 10 μm
Synonym(s):
Latex beads from PS
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| Size/SKU | Availability | Price |
|---|---|---|
5 mL | Check Cart for Availability | $437.00 |
10 mL | Check Cart for Availability | $668.00 |
About This Item
Quality Segment
form
aqueous suspension
crosslinking
2 % cross-linked
concentration
10% (solids)
particle size
10 μm std dev <0.2 μm, coeff var <2%
density
1.05 g/cm3
storage temp.
2-8°C
Analysis Note
For every lot exact values of particle size and standard deviation are determined with an accuracy of 0.01 μm using a Coulter Multisizer.
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This Item | |||
|---|---|---|---|
| description size: 10 μm | description size: 2 μm | description size: 1 μm | description size: 5 μm |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| crosslinking 2 % cross-linked | crosslinking 2 % cross-linked | crosslinking 0 % cross-linked | crosslinking 0 % cross-linked |
| particle size 10 μm std dev <0.2 μm, coeff var <2% | particle size 2 μm std dev <0.05 μm, coeff var <3% | particle size 1 μm std dev <0.1 μm, coeff var <5% | particle size 5 μm std dev <0.1 μm, coeff var <2% |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| concentration 10% (solids) | concentration 10% (solids) | concentration 10% (solids) | concentration 10% (solids) |
| density 1.05 g/cm3 | density 1.05 g/cm3 | density 1.05 g/cm3 | density 1.05 g/cm3 |
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Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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Is this a sterile product and suitable for cell culture and mouse models? How many particles are present per mL?
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What is the number of beads/mL for this product? Thanks
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The number of particles per mL for this product (10 µm) is 1.8 x 10^8.
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Have the particles been counted? If so, how many particles are there in 1mL?
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Based on a solids content of 10 percent, a particle density of 1.05 g/cc, and a particle size of 10.0 microns, this suspension would contain 1.8 X 10E8 particles per mL. Note that if the particle size goes up slightly to 10.3 microns, the value would be 1.7 X 10E8 particles per mL.
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This type of PS microspheres has sulfate groups on its surface due to the synthesis method, which could be considered functional groups that could be applied in DNA-coated applications?
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The beads possess an anionic surface charge due to sulfate groups originating from the initiator in the polymerization reaction. However, these sulfate groups are not suitable for covalent coupling reactions. DNA binding to these particles occurs only through non-specific adsorption, if at all. For DNA extraction, contacting the local technical support team is recommended to explore suitable products. For example, MagPrep® Silica magnetic particles are designed specifically for nucleic acid purification protocols.
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do they react with cells in a solution and do they aggretate together after a time ??
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This product was not assessed for cell activity during formulation. Particles should remain evenly dispersed throughout the suspension. If settling occurs, gentle agitation or vortexing can ensure homogenization.
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Have the PS microspheres undergone surface modification? What functional groups are present, such as carboxyl (-COOH), etc.?
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These microparticles are nominal 10 µm non-functionalized microparticles based on polystyrene, without additional functional groups. The particles only have sulfate groups on their surfaces due to the synthesis method.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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