The stability of this product in solution has not been evaluated. However, the prepared 1.0 mg/mL solution, available as product P4864, is stable for at least 1 year when stored at 2-8°C. Similar storage and stability is expected for this item.
81845
Propidium iodide
≥94% (HPLC), apoptosis marker, powder
Synonym(s):
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
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| Size/SKU | Availability | Price |
|---|---|---|
25 mg | Check Cart for Availability | $92.20 |
100 mg | Check Cart for Availability | $277.00 |
About This Item
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352200
EC Number:
247-081-0
MDL number:
Beilstein/REAXYS Number:
3843838
Assay:
≥94% (HPLC)
Form:
powder
Product Name
Propidium iodide, ≥94% (HPLC)
Quality Segment
assay
≥94% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
fluorescence
λex 482 nm; λem 608 nm (pH 7.2), λex 540 nm; λem 608 nm (bound to DNA)
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
PI is used for selective labelling of dead cells.
Propidium iodide is a fluorescent intercalating agent used for staining. It functions as an apoptosis marker and has linear DNA-binding capacity. Propidium iodide is an effective indicator of tissue injury.
Application
Propidium iodide has been used:
- to stain DNA in cell cycle analysis
- to stain cells and cell nuclei
- for the preparation of propidium iodide (PI)stock solution
- to stain cells for flow cytometry
in scrape-loading dye transfer assay
Features and Benefits
This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
1 of 1
This Item | |||
|---|---|---|---|
| description ≥94% (HPLC) | description A convenient form of propidium iodide useful for flow cytometry studies. | description - | description 1 mg/mL in H2O, ready to use solution, 100 assays |
| form powder | form liquid | form liquid | form liquid |
| assay ≥94% (HPLC) | assay - | assay ≥94% | assay ≥90.0% (HPLC) |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mp 220-225 °C (dec.) (lit.) | mp - | mp - | mp - |
| fluorescence λex 540 nm; λem 608 nm (bound to DNA), λex 482 nm; λem 608 nm (pH 7.2) | fluorescence λex 536 nm, λem 617 nm | fluorescence - | fluorescence λex 530 nm; λem 620 nm±5 nm in 0.1 M phosphate pH 7.0 |
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Signal Word
Warning
Hazard Codes
Hazard Classifications
Eye Irrit. 2 - Muta. 2
Storage Class
11 - Combustible Solids
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
Precautionary Statements
Flash Point (°F)
No data available
Flash Point (°C)
No data available
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