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G3632

Gentamicin sulfate salt

potency: ≥590 I.U. Gentamicin base per mg

Synonym(s):

GM Sulfate, Gentamicin C

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250 mg

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$56.50
1 g

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$124.00
5 g

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$410.00
10 g

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$658.00
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$1,310.00
100 g

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
51281651
eCl@ss:
42020823
NACRES:
NA.76
EC Number:
215-778-9
PubChem Substance ID:

$56.50


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biological source

Micromonospora purpurea

Quality Segment

form

powder

potency

≥590 I.U. Gentamicin base per mg, ≥590 units/mg Gentamicin

storage condition

(Tightly closed. Dry. )

concentration

50 μg/mL in H2O (is the recommended working concentration for eukaryotic cell culture)

color

white to off-white

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycoplasma

application(s)

advanced drug delivery

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

O=S(O)(O)=O.O[C@]1(C)[C@@H]([C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@@](CC[C@H]3N)([C@@H](C)NC)[H])[C@@H](N)C[C@H]2N)OC1)NC.O[C@]4(C)[C@@H]([C@@H](O)[C@@H](O[C@@H]5[C@@H](O)[C@H](O[C@H]6O[C@@](CC[C@H]6N)([C@@H](C)N)[H])[C@@H](N)C[C@H]5N)OC4)NC.O[

InChI

1S/C21H43N5O7.C20H41N5O7.C19H39N5O7.H2O4S/c1-9(25-3)13-6-5-10(22)19(31-13)32-16-11(23)7-12(24)17(14(16)27)33-20-15(28)18(26-4)21(2,29)8-30-20;1-8(21)12-5-4-9(22)18(30-12)31-15-10(23)6-11(24)16(13(15)26)32-19-14(27)17(25-3)20(2,28)7-29-19;1-19(27)7-28-18(13(26)16(19)24-2)31-15-11(23)5-10(22)14(12(15)25)30-17-9(21)4-3-8(6-20)29-17;1-5(2,3)4/h9-20,25-29H,5-8,22-24H2,1-4H3;8-19,25-28H,4-7,21-24H2,1-3H3;8-18,24-27H,3-7,20-23H2,1-2H3;(H2,1,2,3,4)/t9-,10-,11+,12-,13+,14+,15-,16-,17+,18-,19-,20-,21+;8-,9-,10+,11-,12+,13+,14-,15-,16+,17-,18-,19-,20+;8-,9+,10-,11+,12-,13+,14+,15-,16+,17+,18+,19-;/m110./s1

InChI key

RDEIXVOBVLKYNT-HDZPSJEVSA-N

General description

Gentamicin sulfate Salt is a broad-spectrum aminoglycoside antibiotic which is derived from Micromonospora purpurea.

Application

Gentamicin sulfate Salt is used as a selection agent (gentamicin-resistance gene) in cell culture and molecular biology applications. This product is recommended for use at 50 mg/L. Gentamicin sulfate Salt can be used:
  • in the research of the effectiveness of antibiotics against different bacterial cultures
  • in the research of neuronal signal processing

Biochem/physiol Actions

Mode of action: Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site. The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Features and Benefits

High quality antibiotic suitable for mulitple research applications

Preparation Note

Sterile solutions should be stored at 2-8°C. Solutions have also been shown to be stable at room temperature and in boiling aqueous buffers of pH 2-14. [2]

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Freely soluble in water, practically insoluble in ethanol
Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.

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This Item
G1264PHR1077BP174
description

potency: ≥590 I.U. Gentamicin base per mg

description

powder, BioReagent, suitable for cell culture

description

Pharmaceutical Secondary Standard; Certified Reference Material

description

British Pharmacopoeia (BP) Reference Standard

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

-

mode of action

-

antibiotic activity spectrum

mycoplasma, Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

mycoplasma, Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

-

antibiotic activity spectrum

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

-

form

powder

form

powder

form

-

form

powder

application(s)

advanced drug delivery

application(s)

-

application(s)

pharmaceutical (small molecule)
pharmaceutical

application(s)

pharmaceutical
pharmaceutical small molecule

concentration

50 μg/mL in H2O (is the recommended working concentration for eukaryotic cell culture)

concentration

-

concentration

-

concentration

-


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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Certificates of Analysis (COA)

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. G3632の1g当たりの力価について教えて頂きたいです。

    1 answer
    1. Product G3632 potency specification is ≥590 units/mg or I.U. Gentamicin base per mg. For lot specific results, please review the Certificate of Analysis for that lot.

      Helpful?

  4. Regarding the MQ Levels, can you please clarify the difference between "enhanced control" and increased control"

    1 answer
    1. The difference between enhanced control and increased control, in reference to the M-Clarity program, is essentially a matter of degrees. The MQ 100 level products are intended for use in basic research and minimum control is applied to areas such as sourcing, testing method, and available documentation. With increasing MQ levels more rigid controls and guidelines are put in place for these products. The MQ 200 products have an 'increased' level of control while MQ 300 level products having 'enhanced' level of control. Considering the breadth of products offered the specific controls are subject to the nature of the product.

      The link below offers a line-by-line listing of the various discriminating attributes that are considered 'controls'.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/323/519/M-Clarity_attributes_table_ms.pdf

      Helpful?

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