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PPB009
Tris-Borat-EDTA-Puffer
pH 8.3, pHast Pack™, powder
Synonym(e):
1X TBE buffer, TBE buffer, Tris Borate EDTA
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
20 pkg | Ab HEUTE lieferbarvonSchnelldorf | 100,00 € 75,00 € |
Über diesen Artikel
75,00 €
Listenpreis100,00 €Sparen Sie 25%Ab HEUTE lieferbarDetails
product line
pHast Pack™
form
powder
pH
8.3
suitability
suitable for gel electrophoresis
foreign activity
DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
General description
Application
- DNA agarose gel electrophoresis (fragment <1500 bp)
- Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp)
- Denaturing gradient gel electrophoresis
- Northern and Southern blotting
- DNA automated sequencing gel
- Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution)
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Minimize chemical exposure to toxic chemicals
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Preparation Note
Other Notes
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description pH 8.3, pHast Pack™, powder | description BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis | description 5× concentrate, powder blend | description BioReagent, suitable for electrophoresis, 10× concentrate |
| product line pHast Pack™ | product line BioReagent | product line - | product line BioReagent |
| form powder | form powder blend | form powder blend | form solution |
| pH 8.3 | pH 8.1-8.5 (5 ×) | pH 8.1-8.5 (25 °C, 5 ×) | pH - |
| foreign activity DNAse, none detected, Protease, none detected, RNAse, none detected, Nickase, none detected | foreign activity - | foreign activity - | foreign activity - |
| suitability suitable for gel electrophoresis | suitability suitable for electrophoresis | suitability - | suitability suitable for electrophoresis |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
10043-35-3
10043-35-3
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