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PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack, powder, 10X concentrate

Synonym(s):

10X TE buffer


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20 PKG

$85.50

$85.50

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

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product line

pHast Pack

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.9—8.1

solubility

soluble, clear, colorless

cation traces

Fe: <10 ppm

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

storage temp.

room temp

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This Item
PPB009PPB008PPB019
product line

pHast Pack

product line

pHast Pack

product line

pHast Pack

product line

pHast Pack

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

pH

7.9—8.1

pH

8.3

pH

8.3

pH

7.2

storage condition

(Tightly closed. Dry)

storage condition

-

storage condition

-

storage condition

(Tightly closed. Dry)

solubility

soluble, clear, colorless

solubility

-

solubility

-

solubility

-

General description

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Application

TE buffer is intended for use in:
  • Nucleic acid gel electrophoresis
  • storage and dilution of DNA and RNA
  • DNA automated sequencing
  • Immunohistochemistry

Features and Benefits

  • Fast and easy Ready-to-use buffer
  • Minimizes effort - no need to weigh and adjust pH
  • Saves time and eliminates exposure to hazardous chemicals
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouch

Preparation Note

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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