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PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack™, powder

Synonym(s):

1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine

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Size/SKUAvailabilityPrice
20 pkg

Available to ship TODAYfromMILWAUKEE

$116.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

$116.00

Available to ship TODAYDetails

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product line

pHast Pack™

Quality Segment

100

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white to off-white

pH

8.3

solubility

soluble, clear, colorless to faintly yellow

cation traces

Fe: <10 ppm

foreign activity

DNase, none detected, Endonuclease , Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected

storage temp.

room temp

General description

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Application

Suitable for use as:
  • Transfer buffer component in western blot
  • Running buffer in native gel electrophoresis for protein separation
  • Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Saves time and minimizes the effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Tris-Glycine-SDS Buffer 10× Concentrate

description

pH 8.3, pHast Pack™, powder

description

pH 7.2, pHast Pack™, powder

description

Pre-set, pH 8.1, pHast Pack™, powder

description

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

product line

pHast Pack™

product line

pHast Pack™

product line

pHast Pack™

product line

-

form

powder

form

powder

form

powder

form

liquid

pH

8.3

pH

7.2

pH

8.1

pH

-

solubility

soluble, clear, colorless to faintly yellow

solubility

-

solubility

-

solubility

-

storage condition

(Tightly closed. Dry)

storage condition

(Tightly closed. Dry)

storage condition

(Tightly closed. Dry)

storage condition

-

Storage Class

11 - Combustible Solids

wgk

WGK 1

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