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Tris-Glycine Buffer

Name: Tris-Glycine Buffer
Brand: SIAL
Price: 113 USD
Availability: InStock

pH 8.3, pHast Pack^™, powder

Synonym(s):

1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine

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Pack Size	SKU	Availability	Price
20 pkg		Available to ship TODAYfromMILWAUKEE	$113.00

About This Item

NACRES:

NA.25

UNSPSC Code:

41105319

$113.00

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product line

pHast Pack^™

Quality Level

100

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white to off-white

pH

8.3

solubility

soluble, clear, colorless to faintly yellow

cation traces

Fe: <10 ppm

foreign activity

DNase, none detected, Endonuclease , Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected

Related Categories

Biochemicals

Biological Buffers

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Show Differences

1 of 1

This Item	PPB019	PPB023	T7777
Sigma-Aldrich PPB015 Tris-Glycine Buffer	Sigma-Aldrich PPB019 Bis-Tris Bicine EDTA buffer	Sigma-Aldrich PPB023 Tris buffer	Sigma-Aldrich T7777 Tris-Glycine-SDS Buffer 10× Concentrate
product line pHast Pack^™	product line pHast Pack^™	product line pHast Pack^™	product line -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 200
form powder	form powder	form powder	form liquid
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. 2-8°C
storage condition (Tightly closed. Dry)	storage condition (Tightly closed. Dry)	storage condition (Tightly closed. Dry)	storage condition -
pH 8.3	pH 7.2	pH 8.1	pH -

General description

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Application

Suitable for use as:

Transfer buffer component in western blot
Running buffer in native gel electrophoresis for protein separation
Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Features and Benefits

Ready-to-use buffer
No measuring and pH adjusting needed
Saves time and minimizes the effort
Biological tests: free of DNase, RNase, protease, and nickase
Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number

SLCK3077

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Global Trade Item Number

SKU	GTIN
PPB015-20PAK	04061842323258

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