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Nancy-520

Name: Nancy-520
Brand: SIGMA
Price: 198 USD

suitable for RT-PCR, ≥90.0% (HPCE)

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Pack Size	Availability	Price
500 μL	Check Cart for Availability	$198.00
5 mL	Check Cart for Availability	$2,220.00
10 mL	Check Cart for Availability	$3,290.00

About This Item

UNSPSC Code:

12171500

EC Number:

200-664-3

NACRES:

NA.32

$198.00

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Properties

Quality Level

100

assay

≥90.0% (HPCE)

form

liquid

mol wt

78.13 g/mol

greener alternative product characteristics

Designing Safer Chemicals
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color

red to very dark red

bp

189 °C/1,013 hPa

mp

18.4 °C

solubility

DMSO: soluble

density

1.104 g/cm³ at 20 °C

fluorescence

λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA

greener alternative category

Aligned,

storage temp.

2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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This Item	803693	80980	209279
Sigma-Aldrich 01494 Nancy-520	Sigma-Aldrich 803693 Pt/graphene nanocomposite	Sigma-Aldrich 80980 Platinum on activated charcoal	Sigma-Aldrich 209279 Titanium(II) hydride
fluorescence λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA	fluorescence -	fluorescence -	fluorescence -
color red to very dark red	color -	color -	color -
form liquid	form dispersion	form powder	form powder
solubility DMSO: soluble	solubility -	solubility -	solubility -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. -
Quality Level 100	Quality Level -	Quality Level 100	Quality Level 100

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - Solvent

flash_point_c

87 °C - closed cup - Solvent

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Articles

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Global Trade Item Number

SKU	GTIN
CDS007614-500MG	04061828988471
01494-50UL	04061837731846
01494-10ML	04061838609427
01494-5ML	04061838609434
01494-500UL	04061837731839

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