Unfortunately, the stability of this product in solution has not been determined.
10417
8-Anilino-1-naphthalenesulfonic acid ammonium salt
suitable for fluorescence, ≥97.0% (HPLC)
Synonym(s):
1,8-ANS NH4, ANSA, Ammonium 8-anilino-1-naphthalenesulfonate, N-Phenyl peri acid
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Check Cart for Availability | € 51,60 |
25 g | Check Cart for Availability | € 472,00 |
100 g | Check Cart for Availability | € 645,00 |
About This Item
Linear Formula:
C16H13NO3S · NH3
CAS Number:
Molecular Weight:
316.37
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
EC Number:
249-265-6
Beilstein/REAXYS Number:
3581235
MDL number:
Assay:
≥97.0% (HPLC)
Form:
solid
Quality Segment
assay
≥97.0% (HPLC)
form
solid
mol wt
316.37 g/mol
loss
≤2.5% loss on drying
color
faint gray to gray
mp
245-253 °C
solubility
H2O: 0.1 g/5mL, clear to very slightly hazy (hot), NaOH: 1 N, H2O: soluble, acetone: soluble, methanol: soluble
fluorescence
λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA
suitability
suitable for fluorescence
SMILES string
N.OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12
InChI
1S/C16H13NO3S.H3N/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;/h1-11,17H,(H,18,19,20);1H3
InChI key
IPBNQYLKHUNLQE-UHFFFAOYSA-N
Application
This product is an amphiphilic fluorescent probe for protein studies . Excitation of the unbound dye at 380 nm results in a low fluorescent emission with a maximum at 545 nm. The fluorescence intensity of ANS increases when the dye binds to the hydrophobic regions of a protein . The protein-ANS complex has an emission spectrum which is shifted to a broad maximum at 470 nm. At pH 8, protein causes a 40-fold increase in the relative quantum yield compared to free ANS in solution . ANS has been used to monitor protein conformational changes by binding to the hydrophobic regions of a protein , to gain new insight into protein binding interactions, often by acting as reporter or competitor ligands, to investigate the visual excitation process and structural aspects of photoreceptor cell membranes , and to probe (and disrupt) the structure of both high- and low-density lipoproteins. It has also been used as a substrate in a chemiluminescent enzyme immunoassay system and as a dye for yeast viability determination. The conformational states for apo- and holo- yeast alcohol dehydrogenase were reported under conditions of low pH using ANS fluorescence . ANS is also commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of its emission maximum indicates the probe is located in less polar environment
ANS forms an inclusion complex with cyclodextrin. Such model systems are useful to mimic biological recognition and can be studied by measuring the change in fluorescence of free-ANS to complexed-ANS. When ANS enters the hydrophobic core of cyclodestrin, it′s fluorescence increases . Utilized in the reagent phase of a sodium-selective fiber-optic sensor. The reagent phase also contains a copper(II) polyelectrolyte, which binds to ANSA in the absence of sodium and quenches the fluorescence. In the presence of sodium, ANSA forms a cationic complex creating ion-pairs, causing it to fluoresce . ANS is often incorporated into di-block polymers and can be released by changes in the local environment (i.e., temperature, pH, etc.) . ANS is commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media . Fluorescent probe for protein studies using methodologies such as steady-state and dynamic fluorescence measurements .
Fluorescent probe for protein studies.
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This Item | |||
|---|---|---|---|
| description suitable for fluorescence, ≥97.0% (HPLC) | description technical, ≥90% (NT) | description BioXtra, ≥98.0% | description reagent grade, ≥98.0% |
| assay ≥97.0% (HPLC) | assay ≥90% (NT) | assay ≥98.0% | assay ≥98.0% |
| solubility H2O: 0.1 g/5mL, clear to very slightly hazy (hot), H2O: soluble, methanol: soluble, NaOH: 1 N, acetone: soluble | solubility NaOH: 1 N, H2O: soluble, methanol: soluble | solubility H2O: 0.5 M at 20 °C, clear, colorless | solubility water: soluble 132 g/L at 20 °C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| mp 245-253 °C | mp - | mp - | mp 155 °C (dec.) (lit.) |
| form solid | form powder | form powder | form powder or crystals |
| color faint gray to gray | color , Green to Dark Green and Grey to Dark Grey and Green-Grey | color - | color - |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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How long does 8-Anilino-1-naphthalenesulfonic acid ammonium salt (product 10417-5G-F) remain stable after being dissolved in water, and at what temperature should this solution be stored?
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