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14562

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester

BioReagent, suitable for fluorescence

Synonym(s):

BCECF-AM

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Size/SKUAvailabilityPrice
1 mg

Available to ship onSeptember 23, 2026fromMILWAUKEE

$258.00

About This Item

Empirical Formula (Hill Notation):
C39H36O19
Molecular Weight:
808.69
UNSPSC Code:
12352108
NACRES:
NA.32
MDL number:
MFCD01862636

$258.00

Available to ship onSeptember 23, 2026Details

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product line

BioReagent

Quality Segment

form

solid

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

fluorescence

λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

Application

An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues, intercellular spaces, mammalian cells, plant cells, bacteria, and yeast. Also, employed to investigate in assays for cellular functional properties such as adhesion, multi-drug resistance, viability and cytotoxicity, apoptosis and chemotaxis.
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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This Item
B88062188446940
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester BioReagent, suitable for fluorescence

Sigma-Aldrich

14562

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 1 mg/mL in DMSO

Sigma-Aldrich

B8806

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

Sigma-Aldrich

21884

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)

Sigma-Aldrich

46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

description

BioReagent, suitable for fluorescence

description

1 mg/mL in DMSO

description

BioReagent, suitable for fluorescence, ≥85% (HPCE)

description

suitable for fluorescence, ≥75% (HPLC)

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

-

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 490 nm; λem 514 nm (pH 8.2), λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

form

solid

form

liquid

form

-

form

solid

solubility

DMF: soluble, acetonitrile: soluble, DMSO: soluble

solubility

-

solubility

methanol: soluble, DMSO: soluble, DMF: soluble

solubility

DMF: soluble, methanol: soluble

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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