Consult the Butyrate Esterase Test published in the Clinical Microbiology Procedures Handbook. Alternatively, refer to "A Methodology for Detection and Quantification of Esterase Activity" published in Methods in Molecular Biology.
19362
4-Methylumbelliferyl butyrate
lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence
同義詞:
MU-Bu
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經驗公式（希爾表示法）:
C14H14O4
CAS 編號:
分子量：:
246.26
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
241-694-7
MDL number:
Beilstein/REAXYS Number:
227973
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產品名稱
4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)
Quality Segment
assay
≥95% (HPCE)
form
powder or crystals
mp
90-92 °C (lit.)
solubility
DMF: soluble, DMSO: soluble
fluorescence
λex 312 nm in methanol, λex 360 nm; λem 449 nm (Reaction product), λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1
InChI
1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3
InChI key
WKPUJZVCZXWKCK-UHFFFAOYSA-N
Application
suitable as fluorogenic substrate for esterases/lipases
Other Notes
Fluorogenic substrate for butyrate esterase[1]
1 of 1
本產品 | |||
|---|---|---|---|
| assay ≥95% (HPCE) | assay ≥95% (HPCE) | assay ≥98% (TLC) | assay ≥98% (TLC) |
| solubility DMF: soluble, DMSO: soluble | solubility 2-methoxyethanol: soluble, DMSO: soluble, chloroform: soluble | solubility chloroform: 100 mg/mL, clear, colorless | solubility water: 50 mg/mL, clear, colorless to very faintly yellow |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability - |
| form powder or crystals | form solid | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| fluorescence λex 312 nm in methanol, λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase), λex 360 nm; λem 449 nm (Reaction product) | fluorescence λex 312 nm in methanol, λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase) | fluorescence λex 312 nm in methanol, λex 360 nm; λem 499 nm (Reaction product) | fluorescence λex 317 nm; λem 374 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product) |
存儲類別/等級
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I am looking for a general esterase activity protocol using 4-Methylumbelliferyl butyrate as a substrate.
1 answer-
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