DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.
32670
4′,6-Diamidino-2-phenylindole dihydrochloride
suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
Synonym(s):
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Check Cart for Availability | $139.00 |
25 mg | Check Cart for Availability | $574.00 |
About This Item
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
$139.00
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product line
BioReagent
Quality Segment
assay
≥95.0% (HPLC)
form
powder
solubility
DMF: soluble, H2O: soluble
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
suitability
suitable for fluorescence
storage temp.
room temp
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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This Item | |||
|---|---|---|---|
| description suitable for fluorescence, BioReagent, ≥95.0% (HPLC) | description powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence | description Cell-permeable DNA-binding dye. | description 4′,6-Diamidine-2′-phenylindole dihydrochloride |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability - |
| fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA, λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λem ~461 nm, λex ~359 nm | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI) |
| form powder | form powder | form solid | form powder |
| solubility DMF: soluble, H2O: soluble | solubility H2O: 20 mg/mL, PBS: insoluble | solubility ethanol: soluble, water: 5 mg/mL, methanol: soluble | solubility - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
| assay ≥95.0% (HPLC) | assay ≥98% (HPLC) | assay ≥90% (HPLC) | assay >90% |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?
1 answer-
Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C. Immediately examine with a fluorescence microscope.
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How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
1 answer-
At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?
1 answer-
Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.
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