This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
46950
Fluorescein 5(6)-isothiocyanate
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Synonym(s):
FITC, Fluorescein isothiocyanate
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| Size/SKU | Availability | Price |
|---|---|---|
50 mg | Check Cart for Availability | $52.70 |
250 mg | Please contact Customer Service for Availability | $207.00 |
1 g | Please contact Customer Service for Availability | $610.00 |
About This Item
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
Quality Segment
product line
BioReagent
assay
≥90% (HPLC)
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Application
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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This Item | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC) | description ≥90% (HPLC) | description average mol wt 70,000, (FITC:Glucose = 1:250) | description ~20 mg/mL, buffered aqueous solution |
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence - | fluorescence λex 492 nm; λem 518 nm in ethanol | fluorescence - |
| color yellow to dark yellow, orange to dark orange | color yellow to orange | color light yellow to very dark yellow, light orange to very dark orange | color - |
| form powder | form powder | form powder | form buffered aqueous solution |
| solubility acetone: 0.1 g/10 mL, clear to very slightly hazy | solubility acetone: 1 mg/mL, clear, light yellow to yellow | solubility H2O: 50 mg/mL, hazy, orange to very deep orange | solubility - |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay - | assay - |
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Storage Class
11 - Combustible Solids
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
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excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
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Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
Please see the link below to review the Peer Reviewed Publications:
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