Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

46985

Fluorescein-5-thiosemicarbazide

suitable for fluorescence, ~80% (HPCE)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
100 mg

Available to ship TODAYfromMILWAUKEE

$393.00

About This Item

Empirical Formula (Hill Notation):
C21H15N3O5S
CAS Number:
76863-28-0
Molecular Weight:
421.43
NACRES:
NA.32
PubChem Substance ID:
329757284
UNSPSC Code:
12352125
MDL number:
MFCD00155658

$393.00

Available to ship TODAYDetails

​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

assay

~80% (HPCE)

Quality Level

100

form

solid

solubility

DMF: soluble, H2O: soluble

fluorescence

λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

NNC(=S)Nc1ccc(c(c1)C(O)=O)C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24

InChI

1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30)

InChI key

MEUCHQDLZYLNQY-UHFFFAOYSA-N

General description

Fluorescein-5-thiosemicarbazide, also known as FTC, is a fluorescent probe.[1] The FTSC fluorescent spectra is like FITC, a preferred fluorescent tag for proteomics. The fluorescence spectral properties of FTSC-labeled vesicles obtained by reductive amination are fully compatible with the argon laser of fluorescent instruments.[2]

Application

Fluorescein-5-thiosemicarbazide can be used as a fluorescent labeling reagent to label:
  • Cell-surface functional groups (glycophorins) in the study of the effect of deoxygenation in the red blood cell membrane.
  • Saccharides applicable in polysaccharide imaging in live cells.
  • Chondroitin sulfate nanogels applicable in cell-specific drug delivery applications.

Features and Benefits

Fluorescein-5-thiosemicarbazide has the following benefits -

  • It displays strong fluorescence property.[2]
  • Shows an intrinsic reactivity of the thiosemicarbazide group towards the aldehyde group.[2]

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
46950F725021888
Fluorescein-5-thiosemicarbazide suitable for fluorescence, ~80% (HPCE)

Sigma-Aldrich

46985

Fluorescein-5-thiosemicarbazide

Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Sigma-Aldrich

46950

Fluorescein 5(6)-isothiocyanate

Fluorescein isothiocyanate isomer I suitable for protein labeling, ≥90% (HPLC), powder

Sigma-Aldrich

F7250

Fluorescein isothiocyanate isomer I

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

fluorescence

λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0

fluorescence

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

fluorescence

λex 492 nm; λem 518 nm (green)

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

solubility

DMF: soluble, H2O: soluble

solubility

acetone: 0.1 g/10 mL, clear to very slightly hazy

solubility

acetone: 1 mg/mL

solubility

DMF: soluble, DMSO: soluble

form

solid

form

powder

form

powder

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

assay

~80% (HPCE)

assay

≥90% (HPLC)

assay

≥90% (HPLC)

assay

≥90% (HPLC)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN6357
BCCL6736
BCCL1364
BCCJ8072
BCCG3778

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kylan Szeto et al.
PloS one, 8(12), e82667-e82667 (2014-01-01)
Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps. RNA SELEX is more complicated than DNA SELEX because of the additional transcription and reverse transcription steps.
Seiichi Ohta et al.
The Journal of surgical research, 242, 286-295 (2019-05-28)
Adhesion formation is a critical issue in surgery, particularly in hepatectomy. The present study aimed to develop a bilayer adhesion barrier comprising alginate (Alg) of different molecular weight (Mw). It was expected that a slowly dissolving layer remains on the
Injectable hydrogel-incorporated cancer cell-specific cisplatin releasing nanogels for targeted drug delivery
Gil MS, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 5(34), 7140-7152 (2017)
View All Related Papers

Global Trade Item Number

SKUGTIN
46985-100MG-F04061832532141

Questions

Reviews

No rating value

Active Filters