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62309

Lipase from Pseudomonas cepacia

powder, light beige, ≥30 U/mg

Synonym(s):

PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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100 mg

Estimated to ship TODAYfromSAINT LOUIS

$132.00
500 mg

Estimated to ship TODAYfromSAINT LOUIS

$465.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EC Number:
MDL number:
Specific activity:
≥30 U/mg
Biological source:
bacterial (Pseudomonas cepacia)

$132.00


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biological source

bacterial (Pseudomonas cepacia)

Quality Segment

form

powder

specific activity

≥30 U/mg

storage condition

(Tightly closed. Dry)

technique(s)

cell based assay: suitable

color

light beige

solubility

H2O: 2 mg/mL, hazy, faintly yellow

UniProt accession no.

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

General description

Research area: cell-signaling

Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family. Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Pseudomonas cepacian has been used to:
  • catalyze the degradation of polycaprolactone scaffold
  • catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts
  • as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface. Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes. Lipases have broad substrate specificity and high enantioselectivity. This property of lipase makes it a good catalyst in organic synthesis. Lipases play a vital role in fat digestion and metabolism.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)
Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G.
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

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This Item
L8525L1754
description

powder, light beige, ≥30 U/mg

description

lyophilized powder, ≥40,000 units/mg protein

description

Type VII, ≥700 unit/mg solid

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

cell based assay: suitable

specific activity

≥30 U/mg

specific activity

≥40,000 units/mg protein

specific activity

≥700 unit/mg solid

biological source

bacterial (Pseudomonas cepacia)

biological source

fungus (Candida rugosa)

biological source

(from Candida rugosa)

form

powder

form

lyophilized powder

form

lyophilized

solubility

H2O: 2 mg/mL, hazy, faintly yellow

solubility

-

solubility

water: slightly soluble

UniProt accession no.

A0A0J5WKE3

UniProt accession no.

-

UniProt accession no.

-


Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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