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75259

Tris(2-carboxietil)fosfina hydrochloride

BioUltra, ≥98% (NMR)

Sinónimos:

TCEP

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Fórmula empírica (notación de Hill):
C9H15O6P · HCl
Número CAS:
Peso molecular:
286.65
UNSPSC Code:
12352128
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3724376
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product line

BioUltra

Quality Segment

assay

≥98% (NMR)

form

crystals

reaction suitability

reagent type: reductant

loss

≤1.0% loss on drying

pH

1.2-1.5

solubility

H2O: 1 M, clear, colorless (no residue)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.05 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05, λ: 280 nm Amax: 0.03

storage temp.

2-8°C

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution.[1][2] It readily and stoichiometrically reduces disulfides with high specificity.[1] TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.[2]

Application

Reactivo hidrosoluble, utilizado para reducción selectiva de disulfuros. Más estable que el DDT y útil en las aplicaciones de espectrometría de masas.
Tris(2-carboxyethyl)phosphine hydrochloride has been used:
  • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA[3]
  • in reducing sulfhydryl groups to obtain total thiol fraction[4]
  • to reduce protein samples[5]


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Este artículo
68957580560C4706
assay

≥98% (NMR)

assay

97.5-102.5%

assay

≥98% (elemental analysis)

assay

-

product line

BioUltra

product line

BioUltra

product line

-

product line

-

solubility

H2O: 1 M, clear, colorless (no residue)

solubility

H2O: soluble

solubility

water: 5 mg/mL

solubility

H2O: 50 mg/mL

pH

1.2-1.5

pH

-

pH

-

pH

-

form

crystals

form

powder

form

crystalline solid

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C


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pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Clase de almacenamiento

8A - Combustible corrosive hazardous materials

wgk

WGK 1



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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