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94403

Hoechst 33258 solution

1 mg/mL in H2O, ≥98.0% (HPLC)

Synonym(s):

bisBenzimide H 33258 solution

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Pack SizeSKUAvailabilityPrice
1 mL
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$255.00

About This Item

Empirical Formula (Hill Notation):
C25H24N6O · 3HCl
CAS Number:
23491-45-4
Molecular Weight:
533.88
MDL number:
MFCD29074011
UNSPSC Code:
12171500
PubChem Substance ID:
329770334
NACRES:
NA.32
Assay:
≥98.0% (HPLC)
Form:
liquid
Quality level:
100

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Quality Level

100

assay

≥98.0% (HPLC)

form

liquid

mol wt

533.88 g/mol

concentration

1 mg/mL in H2O

color

colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble, H2O: soluble

density

1.00 g/cm3

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. [1]It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.[2]

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA.[3] Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.[4]

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This Item
14530B1155B2883
Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC)

Sigma-Aldrich

94403

Hoechst 33258 solution

bisBenzimide H 33258 suitable for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

14530

bisBenzimide H 33258

bisBenzimide H 33258 powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Sigma-Aldrich

B1155

bisBenzimide H 33258

bisBenzimide H 33258 ≥98% (HPLC)

Sigma-Aldrich

B2883

bisBenzimide H 33258

form

liquid

form

powder

form

powder

form

powder

assay

≥98.0% (HPLC)

assay

≥98.0% (HPLC)

assay

≥98% (HPLC and TLC)

assay

≥98% (HPLC)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

533.88 g/mol

mol wt

-

mol wt

-

mol wt

-

solubility

DMF: soluble, H2O: soluble

solubility

water and ethanol: 10 mg/mL, H2O: soluble, ethanol: soluble

solubility

H2O: 10 mg/mL, water and ethanol: 10 mg/mL

solubility

H2O: 10 mg/mL, water and ethanol: 10 mg/mL, phosphate buffer: precipitates

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9215
BCCN1642
BCCN0204
BCCM2644
BCCL1611

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Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
A Fede et al.
Structure (London, England : 1993), 1(3), 177-186 (1993-11-15)
The chromosomal stain, Hoechst 33258, binds to the minor groove of the DNA double helix and specifically recognizes a run of four A-T base pairs. Extensive biochemical and biophysical studies have been aimed at understanding the binding of the dye
Cornelia Fritsch et al.
PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)
Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
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Global Trade Item Number

SKUGTIN
94403-1ML04061838069023

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