This is not a species specific antigen, it is a slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. It has multi-species reactivity as it is a highly conserved region of the protein among species.
Przejdź do
A5441
Anti-β-Actin (ACTB) Antibody
mouse monoclonal, AC-15
Monoclonal Anti-β-Actin antibody produced in mouse
Synonim(y):
Monoclonal Anti-β-Actin, Anti beta actin monoclonal antibody
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
100 μL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2790,00 zł |
0.2 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2910,00 zł |
0.5 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 5590,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
AC-15, monoclonal
Application:
ELISA (i), IF, IHC (p), WB
Citations:
10789
2790,00 zł
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Pozwól nam pomócNazwa produktu
Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-15, ascites fluid
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
AC-15, monoclonal
mol wt
antigen 42 kDa
contains
15 mM sodium azide
species reactivity
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
should not react with
Dictyostelium discoideum
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts, western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
General description
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Monoclonal Anti-β-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac, and α-smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non-muscle). Actin isoforms show >90% overall sequence homology, but only 50−60% homology in their 18 NH2-terminal residues. The NH2-terminal region of actin appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
Immunogen
slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Application
Monoclonal Anti-β-Actin antibody produced in mouse has also been used:
- in immunofluorescence staining
- in immunoblotting
- in immunohistochemistry
- as a control for protein arrays
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Biochem/physiol Actions
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, cytokinesis, cytoplasmic streaming, secretion and phagocytosis. The actin in cells of various species and tissue origin is very similar in its immunological and physical properties.
Monoclonal Anti β-Actin antibody recognizes an epitope located on the N-terminal end of the β-isoform of actin. The antibody specifically labels β-actin in a wide variety of tissues and species using immunoblotting (42 kDa), immunofluorescent staining of cultured cell lines, and immunohistochemistry.
Physical form
Supplied as ascites fluid with 15mM sodium azide as a preservative.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| Gene Information human ... ACTB(60) | Gene Information human ... ACTB(60) | Gene Information human ... ACTB(60) | Gene Information human ... ACTB(60) |
| clone AC-15, monoclonal | clone AC-74, monoclonal | clone AC-74, monoclonal | clone AC-15, monoclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form ascites fluid | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig | species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human | species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human | species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig |
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Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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species of antigen
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the antibody concentration of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
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Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin?
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This antibody will detect mouse beta actin. When used for western blotting, the secondary antibody may react with mouse IgG from the sample resulting with a bands at 50 kDa and 25 kDa (reducing conditions) or approximately 150 kDa (non-reducing conditions). It is best to use a directly conjugated antibody for immunohistochemistry or immunofluorescent assays. We offer Product numbers A3854, peroxidase conjugated.
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What is the molecular weight of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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Beta Actin has a molecular weight of 42 kDa.
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What IgG light chain (kappa or lambda) is associated with Clone AC-15 for Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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We have not determined the light chain used by monoclonal antibody AC-15. 95% of mouse antibodies have kappa light chains.
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Why do I have 3 bands on my western blot when using Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
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What peroxidase-conjugated secondary antibody should I use with Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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We recommend using Product No. A9044, rabbit anti-mouse IgG, peroxidase conjugate as a secondary antibody. We also offer this same antibody directly conjugated to peroxidase as Product No. A3854.
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