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Anti-phospho-Cofilin (pSer³) antibody produced in rabbit

Name: Anti-phospho-Cofilin (pSer<SUP>3</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 600.1 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-18 kDa phosphoprotein, Anti-CFL1, Anti-Cofilin 1 (non-muscle), Anti-p18

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$706.00 $600.10

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD04040757

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~19 kDa

species reactivity

mouse, human, pig (predicted), rat (predicted)

technique(s)

western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells

UniProt accession no.

P23528

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer3)

Gene Information

human ... CFL1(1072)
mouse ... Cfl1(12631)
rat ... Cfl1(29271)

Description

General description

Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It has gelsolin-like actin filament-severing protein similar to destrin/ADF (Actin Depolymerizing Factor). Cofilin has has a short β-strand at the C terminus. In mammals it has two isoforms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells.

Immunogen

synthetic phosphopeptide corresponding to amino acids 2-9 (pSer³) of human cofilin with a C-terminal added cysteine, conjugated to KLH. This sequence is identical in mouse, rat, and pig.

Application

Anti-phospho-Cofilin antibody was used:

as primary antibody for immunoblottingto to study the role of the Rac1 GTPase.
for western blot analysis in a comparative study of signals induced by integrin ligation during cell attachment, mechanical force from intracellular contraction, or cell stretching by external force.
as primary antibody for western blotting in a study to detect the level of cofilin.

Biochem/physiol Actions

Cofilin binds stoichiometrically to monomeric G-actin and actin protomers in filaments in an apparently pH-dependent, Ca²⁺ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist.It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends under specific conditions. Cofilin is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. It′s activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it behaves inactive and unable to bond with actin. Phosphorylation of cofilin is regulated in vertebrates by at least four protein kinases: LIM Kinase 1, LIM Kinase 2, Testicular Kinase 1, and Testicular Kinase 2. The dephosphorylation of cofilin enables its actin severing and depolymerizing activity and drives directional cell motility, thus providing a simple phosphoregulatory mechanism for actin reorganization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4500148	WH0001073M3	P0101
$Anti-phospho-Cofilin (pSer3) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C8992 Anti-phospho-Cofilin (pSer³) antibody produced in rabbit	Sigma-Aldrich SAB4500148 Anti-Cofilin antibody produced in rabbit	Sigma-Aldrich WH0001073M3 Monoclonal Anti-CFL2 antibody produced in mouse	$Anti-Profilin 2 (C-terminal) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich P0101 Anti-Profilin 2 (C-terminal) antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form IgG fraction of antiserum
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
technique(s) western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells	technique(s) ELISA: 1:40000, western blot: 1:500-1:1000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL	technique(s) western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain
UniProt accession no. P23528	UniProt accession no. P23528	UniProt accession no. Q9Y281	UniProt accession no. P35080
mol wt antigen ~19 kDa	mol wt antigen 18 kDa	mol wt -	mol wt antigen ~14 kDa

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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