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Anti-DHFR, N-terminal antibody produced in rabbit

Name: Anti-DHFR, N-terminal antibody produced in rabbit
Brand: SIGMA
Price: 341.7 EUR

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Dihydrofolate reductase

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Size/SKU	Availability	Price
100 μg	Please contact Customer Service for Availability	€402.00 €341.70

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD05665212

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB CL

Citations:

€341.70

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR, western blot (chemiluminescent): 0.5-1.0 μg/mL using 100 ng of purified recombinant DHFR

UniProt accession no.

P00374

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Description

General description

Dihydrofolate reductase has 187 amino acids and corresponds to molecular weight of 18-20 kDa. It is mapped to chromosome 5q22 region. DHFR contains substrate binding site and coenzyme binding domain.

Immunogen

The immunizing sequence is conserved in mouse, human and rat.

Application

Anti-DHFR, N-terminal antibody produced in rabbit has been used in immunoprecipitation and western blotting.

Biochem/physiol Actions

Anti-DHFR, N-terminal antibody is specific for the epitope residing within amino acids of mouse DHFR. The antibody interacts with DHFR and DHFR fusion proteins. The product is specific for DHFR in humans, mice and rats. Staining of the DHFR band by immunoblotting is inhibited by the immunizing peptide.

Dihydrofolate reductase (DHFR) inhibitors, such as methotrexate, are folate analogs which can bind to the active site and deactivate the enzyme. From another angle, structural and enzymatic properties of DHFR led to development of a variety of screenings, in which DHFR can function in a fashion that resembles reporter genes. The screenings are based on the fact that DHFR can be dissected into two halves that can reassemble to form an active enzyme. Thus, each of the two halves of DHFR can be expressed as two fusion proteins that when interacting with each other can restore the DHFR enzymatic activity. The readout of this protein-protein interaction and consequent enzymatic activity, can be either restoration of growth in bacterial, yeast and plant, or receptor activation. The approach was proved to be useful for receptor-ligand, antigen-antibody and other interactions.

Dihydrofolate reductase (DHFR) is an NADPH dependent enzyme that reduces dihydrofolate and regenerates tetrahydrofolate. DHFR is involved in the biosynthesis of thymidylate and purines, and thereby regulates DNA synthesis and cell survival. Alterations in DHFR have been associated with impaired nucleic acid synthesis and cell death. Thus, this enzyme has important therapeutic implications in cell proliferative disorders such as cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R8656	SAB4200528	N9414
Sigma-Aldrich D1067 Anti-DHFR, N-terminal antibody produced in rabbit	Sigma-Aldrich R8656 Anti-Peroxiredoxin 2 (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB4200528 Anti-HMGCR antibody produced in rabbit	Sigma-Aldrich N9414 Anti-NUCB2 (N-terminal) antibody produced in rabbit
description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody	description ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level -	Quality Level -
species reactivity mouse, human, rat	species reactivity rat, human, mouse	species reactivity human	species reactivity rat, mouse
technique(s) immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR, western blot (chemiluminescent): 0.5-1.0 μg/mL using 100 ng of purified recombinant DHFR	technique(s) immunoprecipitation (IP): 2.0-5.0 μg using lysate of mouse 3T3 cells, western blot: 0.5-1.0 using whole extract of human HeLa cells, western blot: 1-2 μg/mL using whole extract of rat NRK cells	technique(s) indirect immunofluorescence: 1.0-2.0 μg/mL using mevastatin-treated HepG2 cells., western blot: 1.5-3.0 μg/mL using extracts of mevastatin-treated HepG2 cells.	technique(s) indirect immunofluorescence: 1-2 μg/mL using Rat2 cells, western blot: 1.5-3.0 μg/mL using Rat2 cell lysates, western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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