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Anti-Derlin-2 antibody produced in rabbit

Name: Anti-Derlin-2 antibody produced in rabbit
Brand: SIGMA
Price: 469.2 USD
Availability: InStock

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DERL2, Anti-Der1-like domain family, member 2

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00 $469.20

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

$469.20

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~21 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using HeLa cells, western blot: 2.0-4.0 μg/mL using whole extract of mouse 3T3 cells

UniProt accession no.

Q9GZP9

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DERL2(51009)
mouse ... Derl2(116891)

Description

General description

Derlin-1, Derlin-2, and Derlin-3 are the mammalian homologues of yeast Der1p, a transmembrane protein required for yeast endoplasmic reticulum-associated degradation (ERAD). Derlin-2 is approximately 30% identical to Derlin-1. In rat Derlin-2 is present to the endoplasmic reticulum(ER) membrane and forms a multisubunit complex with other proteins.

Immunogen

synthetic peptide corresponding to amino acid residues 223-239 of human derlin-2 conjugated to KLH via an N-terminal added cysteine residue. The corresponding sequence is identical in mouse.

Application

Anti-Derlin-2 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Derlin-2, also known as F-LANa, is involved in the degradation of misfolded glycoproteins in the ER. Derlin-2 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-2 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200148	R4407	HPA048957
Sigma-Aldrich D1194 Anti-Derlin-2 antibody produced in rabbit	Sigma-Aldrich SAB4200148 Anti-Derlin-1 antibody, Mouse monoclonal	Sigma-Aldrich R4407 Anti-RER1 antibody produced in rabbit	Sigma-Aldrich HPA048957 Anti-DERL2 antibody produced in rabbit
description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone Derlin1-1, purified from hybridoma cell culture	description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone polyclonal	clone Derlin1-1, monoclonal	clone polyclonal	clone polyclonal
technique(s) western blot: 2.0-4.0 μg/mL using whole extract of mouse 3T3 cells, indirect immunofluorescence: 5-10 μg/mL using HeLa cells	technique(s) western blot: 2-4 μg/mL using whole extracts of mouse NIH-3T3 or rat NRK cells, immunoprecipitation (IP): suitable	technique(s) western blot: 3-6 μg/mL using whole extract of HEK-293T cells expressing human Rer1, indirect immunofluorescence: 1-2 μg/mL using mouse 3T3 and rat NRK cells	technique(s) immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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